biostudies-literatureSieniawska ENarodowe Centrum NaukiE256https://www.ebi.ac.uk/biostudies/studies/S-EPMC6017462biostudies-literatureUnknown23(2)The most commonly used plant source of β-elemene is Curcuma wenyujin Y. H. Chen & C. Ling (syn. of Curcuma aromatic Salisb.) with its content in supercritical CO₂ extract up to 27.83%. However, the other rich source of this compound is Nigella damascena L. essential oil, in which β-elemene accounts for 47%. In this work, the effective protocol for preparative isolation of β-elemene from a new source-N. damascena essential oil-using high performance counter-current chromatography HPCCC was elaborated. Furthermore, since sesquiterpens are known as potent antimicrobials, the need for finding new agents designed to combat multi-drug resistant strains was addressed and the purified target compound and the essential oil were tested for its activity against a panel of Gram-positive and Gram-negatMolecules (Basel, Switzerland)Nigella damascena L. Essential Oil-A Valuable Source of β-Elemene for Antimicrobial Testing.PMC60174622013/11/D/NZ7/01613Golus JSkalicka-Wozniak KGinalska GSieniawska ESawicki RSwatko-Ossor MfalseNigella damascena L. Essential Oil-A Valuable Source of β-Elemene for Antimicrobial Testing.The most commonly used plant source of β-elemene is Curcuma wenyujin Y. H. Chen & C. Ling (syn. of Curcuma aromatic Salisb.) with its content in supercritical CO₂ extract up to 27.83%. However, the other rich source of this compound is Nigella damascena L. essential oil, in which β-elemene accounts for 47%. In this work, the effective protocol for preparative isolation of β-elemene from a new source-N. damascena essential oil-using high performance counter-current chromatography HPCCC was elaborated. Furthermore, since sesquiterpens are known as potent antimicrobials, the need for finding new agents designed to combat multi-drug resistant strains was addressed and the purified target compound and the essential oil were tested for its activity against a panel of Gram-positive and Gram-negat2018-01-01T00:00:00Z2018 Jan2025-04-18T15:22:37.226Z2019-03-27T00:06:37ZS-EPMC60174622938209710.3390/molecules23020256