biostudies-literatureWebster CGPlant Health and Environment Division of INRAFrench National Research AgencyBill and Melinda Gates FoundationRegion LRe00432-18https://www.ebi.ac.uk/biostudies/studies/S-EPMC6026765biostudies-literatureUnknown92(14)Plant viruses transmitted by insects cause tremendous losses in most important crops around the world. The identification of receptors of plant viruses within their insect vectors is a key challenge to understanding the mechanisms of transmission and offers an avenue for future alternative control strategies to limit viral spread. We here report the identification of two cuticular proteins within aphid mouthparts, and we provide experimental support for the role of one of them in the transmission of a noncirculative virus. These two proteins, named Stylin-01 and Stylin-02, belong to the RR-1 cuticular protein subfamily and are highly conserved among aphid species. Using an immunolabeling approach, they were localized in the maxillary stylets of the pea aphid <i>Acyrthosiphon pisum</i> and Journal of virologyIdentification of Plant Virus Receptor Candidates in the Stylets of Their Aphid Vectors.PMC6026765ANR15-CE20-0011OPP1130147Perry KLThompson JRvan Munster MWebster CGUzest MPichon ECalevro FFereres AMoreno ABlanc SGargani DDeshoux MKrenz BJimenez JMonsion BfalseIdentification of Plant Virus Receptor Candidates in the Stylets of Their Aphid Vectors.Plant viruses transmitted by insects cause tremendous losses in most important crops around the world. The identification of receptors of plant viruses within their insect vectors is a key challenge to understanding the mechanisms of transmission and offers an avenue for future alternative control strategies to limit viral spread. We here report the identification of two cuticular proteins within aphid mouthparts, and we provide experimental support for the role of one of them in the transmission of a noncirculative virus. These two proteins, named Stylin-01 and Stylin-02, belong to the RR-1 cuticular protein subfamily and are highly conserved among aphid species. Using an immunolabeling approach, they were localized in the maxillary stylets of the pea aphid <i>Acyrthosiphon pisum</i> and 2018-01-01T00:00:00Z2018 Jul2026-05-02T17:36:16.888Z2019-03-26T23:45:15ZS-EPMC60267652976933210.1128/JVI.00432-18