{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Kok YL"],"funding":["Swiss National Science Foundation","Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung"],"pagination":["10204"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6033903"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["8(1)"],"pubmed_abstract":["Long-lived latently HIV-1-infected cells represent a barrier to cure. We developed a dual-fluorescence HIV-1-based vector containing a pair of genetic insulators flanking a constitutive fluorescent reporter gene to study HIV-1 latency. The protective effects of these genetic insulators are demonstrated through long-term (up to 394 days) stable fluorescence profiles in transduced SUP-T1 cells. Analysis of 1,941 vector integration sites confirmed reproduction of HIV-1 integration patterns. We sorted monoclonal cells representing latent HIV-1 infections and found that both vector integration sites and integrity of the vector genomes influence the reactivation potentials of latent HIV-1 promoters. Interestingly, some latent monoclonal cells exhibited a small cell subpopulation with a spontaneo"],"journal":["Scientific reports"],"pubmed_title":["Spontaneous reactivation of latent HIV-1 promoters is linked to the cell cycle as revealed by a genetic-insulators-containing dual-fluorescence HIV-1-based vector."],"pmcid":["PMC6033903"],"funding_grant_id":["141067","310030_141067","310030"],"pubmed_authors":["Berens C","Metzner KJ","Kok YL","Kelley A","Kouyos RD","Inderbitzin A","Neumann K","Jorimann L","Shilaih M","Schmutz S","Vongrad V","Gunthard HF"],"additional_accession":[]},"is_claimable":false,"name":"Spontaneous reactivation of latent HIV-1 promoters is linked to the cell cycle as revealed by a genetic-insulators-containing dual-fluorescence HIV-1-based vector.","description":"Long-lived latently HIV-1-infected cells represent a barrier to cure. We developed a dual-fluorescence HIV-1-based vector containing a pair of genetic insulators flanking a constitutive fluorescent reporter gene to study HIV-1 latency. The protective effects of these genetic insulators are demonstrated through long-term (up to 394 days) stable fluorescence profiles in transduced SUP-T1 cells. Analysis of 1,941 vector integration sites confirmed reproduction of HIV-1 integration patterns. We sorted monoclonal cells representing latent HIV-1 infections and found that both vector integration sites and integrity of the vector genomes influence the reactivation potentials of latent HIV-1 promoters. Interestingly, some latent monoclonal cells exhibited a small cell subpopulation with a spontaneo","dates":{"release":"2018-01-01T00:00:00Z","publication":"2018 Jul","modification":"2026-05-05T20:51:21.505Z","creation":"2019-03-26T23:45:59Z"},"accession":"S-EPMC6033903","cross_references":{"pubmed":["29977044"],"doi":["10.1038/s41598-018-28161-y"]}}