biostudies-literatureUnknown12(8)Chang JMethanotrophs synthesize methanobactin, a secondary metabolite that binds copper with an unprecedentedly high affinity. Such a strategy may provide methanotrophs a "copper monopoly" that can inhibit the activity of copper-containing enzymes of other microbes, e.g., copper-dependent N₂O reductases. Here, we show that methanobactin from Methylosinus trichosporium OB3b inhibited N₂O reduction in denitrifiers. When Pseudomonas stutzeri DCP-Ps1 was incubated in cocultures with M. trichosporium OB3b or with purified methanobactin from M. trichosporium OB3b, stoichiometric N₂O production was observed from NO₃^- reduction, whereas no significant N₂O accumulation was observed in cocultures with a mutant defective in methanobactin prodThe ISME journal2086-2089https://www.ebi.ac.uk/biostudies/studies/S-EPMC6052078biostudies-literatureMethanobactin from Methylosinus trichosporium OB3b inhibits N₂O reduction in denitrifiers.PMC6052078DiSpirito AAGu WYoon SChang JSemrau JDPark DfalseMethanobactin from Methylosinus trichosporium OB3b inhibits N₂O reduction in denitrifiers.Methanotrophs synthesize methanobactin, a secondary metabolite that binds copper with an unprecedentedly high affinity. Such a strategy may provide methanotrophs a "copper monopoly" that can inhibit the activity of copper-containing enzymes of other microbes, e.g., copper-dependent N₂O reductases. Here, we show that methanobactin from Methylosinus trichosporium OB3b inhibited N₂O reduction in denitrifiers. When Pseudomonas stutzeri DCP-Ps1 was incubated in cocultures with M. trichosporium OB3b or with purified methanobactin from M. trichosporium OB3b, stoichiometric N₂O production was observed from NO₃^- reduction, whereas no significant N₂O accumulation was observed in cocultures with a mutant defective in methanobactin prod2018-01-01T00:00:00Z2018 Aug2026-05-05T23:12:53.447Z2019-08-07T07:01:11ZS-EPMC60520782933053210.1038/s41396-017-0022-8