biostudies-literatureAdar CEuropean Research CouncilIsrael Science Foundation (ISF)11046https://www.ebi.ac.uk/biostudies/studies/S-EPMC6056459biostudies-literatureUnknown8(1)Ice-binding proteins (IBPs) permit their hosts to thrive in the presence of ice. The ability of IBPs to control ice growth makes them potential additives in industries ranging from food storage and cryopreservation to anti-icing systems. For IBPs to be used in commercial applications, however, methods are needed to produce sufficient quantities of high-quality proteins. Here, we describe a new method for IBP purification, termed falling water ice affinity purification (FWIP). The method is based on the affinity of IBPs for ice and does not require molecular tags. A crude IBP solution is allowed to flow over a chilled vertical surface of a commercial ice machine. The temperature of the surface is lowered gradually until ice crystals are produced, to which the IBPs bind but other solutes do Scientific reportsFalling water ice affinity purification of ice-binding proteins.PMC6056459930/16281595Friehmann TSirotinskaya VAdar CBar Dolev MBraslavsky IfalseFalling water ice affinity purification of ice-binding proteins.Ice-binding proteins (IBPs) permit their hosts to thrive in the presence of ice. The ability of IBPs to control ice growth makes them potential additives in industries ranging from food storage and cryopreservation to anti-icing systems. For IBPs to be used in commercial applications, however, methods are needed to produce sufficient quantities of high-quality proteins. Here, we describe a new method for IBP purification, termed falling water ice affinity purification (FWIP). The method is based on the affinity of IBPs for ice and does not require molecular tags. A crude IBP solution is allowed to flow over a chilled vertical surface of a commercial ice machine. The temperature of the surface is lowered gradually until ice crystals are produced, to which the IBPs bind but other solutes do 2018-01-01T00:00:00Z2018 Jul2025-04-04T23:44:15.867Z2019-03-26T23:48:18ZS-EPMC60564593003821210.1038/s41598-018-29312-x