biostudies-literatureUnknown9Horvat SDisturbance in gut microbiota is crucial for the development of <i>Clostridioides difficile</i> infection (CDI). Different mechanisms through which gut microbiota influences <i>C. difficile</i> colonization are known. However, <i>C. difficile</i> could also affect gut microbiota balance as previously demonstrated by cultivation of fecal microbiota in <i>C. difficile</i> conditioned medium. In current study, the interactions of <i>C. difficile</i> cells with gut microbiota were addressed. Three different strains (ribotypes 027, 014/020, and 010) were co-cultivated with two types of fecal microbiota (healthy and dysbiotic) using <i>in vitro</i> batch model. While all strains showed higher sporulation frequency in the presence of dysbiotic fecal microbiota, the growth was strain dependent. <i>C. difficile</i> either proliferated to comparable levels in the presence of dysbiotic and healthy fecal microbiota or grew better in co-culture with dysbiotic microbiota. In co-cultures with any <i>C. difficile</i> strain fecal microbiota showed decreased richness and diversity. Dysbiotic fecal microbiota was more affected after co-culture with <i>C. difficile</i> than healthy microbiota. Altogether, 62 OTUs were significantly changed in co-cultures of dysbiotic microbiota/<i>C. difficile</i> and 45 OTUs in co-cultures of healthy microbiota/<i>C. difficile</i>. However, the majority of significantly changed OTUs in both types of microbiota belonged to the phylum <i>Firmicutes</i> with <i>Lachnospiraceae</i> and <i>Ruminococcaceae</i> origin.Frontiers in microbiology1633https://www.ebi.ac.uk/biostudies/studies/S-EPMC6066498biostudies-literatureInteractions Between <i>Clostridioides difficile</i> and Fecal Microbiota in <i>in Vitro</i> Batch Model: Growth, Sporulation, and Microbiota Changes.PMC6066498Rupnik MHorvat SfalseInteractions Between <i>Clostridioides difficile</i> and Fecal Microbiota in <i>in Vitro</i> Batch Model: Growth, Sporulation, and Microbiota Changes.Disturbance in gut microbiota is crucial for the development of <i>Clostridioides difficile</i> infection (CDI). Different mechanisms through which gut microbiota influences <i>C. difficile</i> colonization are known. However, <i>C. difficile</i> could also affect gut microbiota balance as previously demonstrated by cultivation of fecal microbiota in <i>C. difficile</i> conditioned medium. In current study, the interactions of <i>C. difficile</i> cells with gut microbiota were addressed. Three different strains (ribotypes 027, 014/020, and 010) were co-cultivated with two types of fecal microbiota (healthy and dysbiotic) using <i>in vitro</i> batch model. While all strains showed higher sporulation frequency in the presence of dysbiotic fecal microbiota, the growth was strain dependent. <i>C. difficile</i> either proliferated to comparable levels in the presence of dysbiotic and healthy fecal microbiota or grew better in co-culture with dysbiotic microbiota. In co-cultures with any <i>C. difficile</i> strain fecal microbiota showed decreased richness and diversity. Dysbiotic fecal microbiota was more affected after co-culture with <i>C. difficile</i> than healthy microbiota. Altogether, 62 OTUs were significantly changed in co-cultures of dysbiotic microbiota/<i>C. difficile</i> and 45 OTUs in co-cultures of healthy microbiota/<i>C. difficile</i>. However, the majority of significantly changed OTUs in both types of microbiota belonged to the phylum <i>Firmicutes</i> with <i>Lachnospiraceae</i> and <i>Ruminococcaceae</i> origin.2018-01-01T00:00:00Z20182022-02-10T15:07:30.626Z2019-03-26T23:50:22ZS-EPMC60664983008766010.3389/fmicb.2018.01633