biostudies-literatureUnknown20(8)Gow CHA kinesin family member 5b (KIF5B)-MET proto-oncogene, receptor tyrosine kinase (MET) rearrangement was reported in patients with lung adenocarcinoma but its oncogenic function was not fully evaluated. We used one-step reverse transcription-polymerase chain reaction for RNA samples to screen for the KIF5B-MET fusion in 206 lung adenocarcinoma and 28 pulmonary sarcomatoid carcinoma patients. Genomic breakpoints of KIF5B-MET were determined by targeted next-generation sequencing. Soft agar colony formation assays, proliferation assays, and a xenograft mouse model were used to investigate its oncogenic activity. In addition, specific MET inhibitors were administered to evaluate their anti-tumor activities. A KIF5B-MET fusion variant in a patient with a mixed-type adenocarcinoma and sarcomatoiNeoplasia (New York, N.Y.)838-847https://www.ebi.ac.uk/biostudies/studies/S-EPMC6068088biostudies-literatureOncogenic Function of a KIF5B-MET Fusion Variant in Non-Small Cell Lung Cancer.PMC6068088Liu YNTsai THChen PLHsieh MSChang SHLi HYGow CHTsai MFLuo SCShih JYfalseOncogenic Function of a KIF5B-MET Fusion Variant in Non-Small Cell Lung Cancer.A kinesin family member 5b (KIF5B)-MET proto-oncogene, receptor tyrosine kinase (MET) rearrangement was reported in patients with lung adenocarcinoma but its oncogenic function was not fully evaluated. We used one-step reverse transcription-polymerase chain reaction for RNA samples to screen for the KIF5B-MET fusion in 206 lung adenocarcinoma and 28 pulmonary sarcomatoid carcinoma patients. Genomic breakpoints of KIF5B-MET were determined by targeted next-generation sequencing. Soft agar colony formation assays, proliferation assays, and a xenograft mouse model were used to investigate its oncogenic activity. In addition, specific MET inhibitors were administered to evaluate their anti-tumor activities. A KIF5B-MET fusion variant in a patient with a mixed-type adenocarcinoma and sarcomatoi2018-01-01T00:00:00Z2018 Aug2025-04-04T20:30:45.843Z2019-03-26T23:49:11ZS-EPMC60680883001515910.1016/j.neo.2018.06.007