biostudies-literatureKaletsch ADeutsche ForschungsgemeinschaftForschungskommission der Medizinischen Fakultät der HHUDr.-Brigitte- &amp;Constanze-Wegener-Stiftung100https://www.ebi.ac.uk/biostudies/studies/S-EPMC6069857biostudies-literatureUnknown10(1)<h4>Background</h4>Histone deacetylase inhibitors (HDACi) are promising anti-cancer drugs that could also be employed for urothelial carcinoma (UC) therapy. It is unclear, however, whether inhibition of all 11 zinc-dependent HDACs or of individual enzymes is more efficacious and specific. Here, we investigated the novel HDACi 19i (LMK235) with presumed preferential activity against class IIA HDAC4/5 in comparison to the pan-HDACi vorinostat (SAHA) and the HDAC4-specific HDACi TMP269 in UC cell lines with basal expression of HDAC4 and characterized two HDAC4-overexpressing UC cell lines.<h4>Methods</h4>Cytotoxic concentrations 50% (CC₅₀s) for HDACi were determined by MTT assay and high-content analysis-based fluorescent live/dead assay in UC cell lines with different expression oClinical epigeneticsEffects of novel HDAC inhibitors on urothelial carcinoma cells.PMC60698571142/2015NI 1398/1-1Schulz WAHansen FKKassack MUPinkerneil MWang CKurz TNiegisch GGohlke HKaletsch AHanenberg HHoffmann MJGertzen CWiek CJaguva Vasudevan AAfalseEffects of novel HDAC inhibitors on urothelial carcinoma cells.<h4>Background</h4>Histone deacetylase inhibitors (HDACi) are promising anti-cancer drugs that could also be employed for urothelial carcinoma (UC) therapy. It is unclear, however, whether inhibition of all 11 zinc-dependent HDACs or of individual enzymes is more efficacious and specific. Here, we investigated the novel HDACi 19i (LMK235) with presumed preferential activity against class IIA HDAC4/5 in comparison to the pan-HDACi vorinostat (SAHA) and the HDAC4-specific HDACi TMP269 in UC cell lines with basal expression of HDAC4 and characterized two HDAC4-overexpressing UC cell lines.<h4>Methods</h4>Cytotoxic concentrations 50% (CC₅₀s) for HDACi were determined by MTT assay and high-content analysis-based fluorescent live/dead assay in UC cell lines with different expression o2018-01-01T00:00:00Z2018 Jul2025-04-04T20:30:53.606Z2019-03-26T23:49:58ZS-EPMC60698573006450110.1186/s13148-018-0531-y