biostudies-literatureTyurin APRussian Academy of SciencesNational Natural Science Foundation of ChinaRussian Foundation for Basic Research667-675https://www.ebi.ac.uk/biostudies/studies/S-EPMC6071864biostudies-literatureUnknown9(4)The study of an archived sample of crystallomycin complex using HPLC, ESI HRMS, and 2D NMR showed that two major components of the antibiotic, compounds <b>1</b> and <b>2</b>, are lipopeptides having the same peptide core, Asp1-cyclo(Dab2-Pip3-MeAsp4-Asp5-Gly6-Asp7-Gly8-Dab9-Val10-Pro11-), <i>N</i>-acylated either with Δ³-iso-tetradecenoyl or Δ³-anteiso-pentadecenoyl that are identical to aspartocins C and B, respectively. According to the 2D NMR study, compound <b>2</b> in DMSO solution exists as a mixture of four conformers. The producing strain was identified as <i>Streptomyces griseorubens</i>. Compounds <b>1</b> and <b>2</b> have considerable Ca²⁺-dependent activity against Gram-positive bacteria including five MRSA strains.MedChemCommCrystallomycin revisited after 60 years: aspartocins B and C.PMC60718648161153071617-53-53130Grammatikova NEEfimenko TAShuvalov MVMalanicheva IASun CLiu SShenkarev ZOProkhorenko IAKorshun VATyurin APParamonov ASSolyev PNAlferova VAEfremenkova OVTerekhova LPfalseCrystallomycin revisited after 60 years: aspartocins B and C.The study of an archived sample of crystallomycin complex using HPLC, ESI HRMS, and 2D NMR showed that two major components of the antibiotic, compounds <b>1</b> and <b>2</b>, are lipopeptides having the same peptide core, Asp1-cyclo(Dab2-Pip3-MeAsp4-Asp5-Gly6-Asp7-Gly8-Dab9-Val10-Pro11-), <i>N</i>-acylated either with Δ³-iso-tetradecenoyl or Δ³-anteiso-pentadecenoyl that are identical to aspartocins C and B, respectively. According to the 2D NMR study, compound <b>2</b> in DMSO solution exists as a mixture of four conformers. The producing strain was identified as <i>Streptomyces griseorubens</i>. Compounds <b>1</b> and <b>2</b> have considerable Ca²⁺-dependent activity against Gram-positive bacteria including five MRSA strains.2018-01-01T00:00:00Z2018 Apr2026-04-30T23:04:10.382Z2019-06-06T19:56:37ZS-EPMC60718643010895710.1039/c8md00002f