biostudies-literatureUnknown9(55)de Vroome SWProteins are routinely measured in clinical laboratories for diagnosis, prognosis and therapy monitoring. Nevertheless, both test improvements (performance) and innovations (biomarkers) are needed, and protein <i>N</i>-glycosylation offers a rich source of potential markers. Here, we have analyzed the total serum <i>N</i>-glycome in a matched case-control study (124 cases versus 124 controls) of colorectal cancer patients. The results were validated in an independent sample cohort (both 61 cases versus 61 controls) and further tested in post-operative samples of cured patients. Our results revealed significant differences between patients and controls, with increased size (antennae) and sialylation of the <i>N</i>-glycans in the colorectal cancer patient sera as compared to mainly di-antenOncotarget30610-30623https://www.ebi.ac.uk/biostudies/studies/S-EPMC6078140biostudies-literatureSerum <i>N</i>-glycome alterations in colorectal cancer associate with survival.PMC6078140Mesker WEvan der Burgt YEMGirondo MRHolst Sde Vroome SWTollenaar RAEMWuhrer MfalseSerum <i>N</i>-glycome alterations in colorectal cancer associate with survival.Proteins are routinely measured in clinical laboratories for diagnosis, prognosis and therapy monitoring. Nevertheless, both test improvements (performance) and innovations (biomarkers) are needed, and protein <i>N</i>-glycosylation offers a rich source of potential markers. Here, we have analyzed the total serum <i>N</i>-glycome in a matched case-control study (124 cases versus 124 controls) of colorectal cancer patients. The results were validated in an independent sample cohort (both 61 cases versus 61 controls) and further tested in post-operative samples of cured patients. Our results revealed significant differences between patients and controls, with increased size (antennae) and sialylation of the <i>N</i>-glycans in the colorectal cancer patient sera as compared to mainly di-anten2018-01-01T00:00:00Z2018 Jul2026-07-15T20:58:07.843Z2019-03-26T23:50:52ZS-EPMC60781403009397310.18632/oncotarget.25753