{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Weinert BT"],"funding":["FAMRI","Novo Nordisk Foundation","Novo Nordisk","Novo Nordisk Foundation Center for Protein Research","Danish National Research Foundation (DNRF)","Novo Nordisk Foundation Section for Basic Stem Cell Biology","NIH","Novo Nordisk Fonden","NIGMS NIH HHS"],"pagination":["231-244.e12"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6078418"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["174(1)"],"pubmed_abstract":["The acetyltransferases CBP and p300 are multifunctional transcriptional co-activators. Here, we combined quantitative proteomics with CBP/p300-specific catalytic inhibitors, bromodomain inhibitor, and gene knockout to reveal a comprehensive map of regulated acetylation sites and their dynamic turnover rates. CBP/p300 acetylates thousands of sites, including signature histone sites and a multitude of sites on signaling effectors and enhancer-associated transcriptional regulators. Time-resolved acetylome analyses identified a subset of CBP/p300-regulated sites with very rapid (<30 min) acetylation turnover, revealing a dynamic balance between acetylation and deacetylation. Quantification of acetylation, mRNA, and protein abundance after CBP/p300 inhibition reveals a kinetically competent net"],"journal":["Cell"],"pubmed_title":["Time-Resolved Analysis Reveals Rapid Dynamics and Broad Scope of the CBP/p300 Acetylome."],"pmcid":["PMC6078418"],"funding_grant_id":["K99 GM124357","R37 GM062437","NNF14CC0001","GM62437","116","PI Chunaram Choudhary","NNF15OC0017774","Brickman group NNF","NNF14OC0008541"],"pubmed_authors":["Choudhary C","Cole PA","Srinivasan B","Hamilton WB","Lai A","Zucconi BE","Weinert BT","Liu WR","Narita T","Kesicki EA","Satpathy S","Scholz C","Bromberg KD","Wang WW","Brickman JM","Hansen BK"],"additional_accession":[]},"is_claimable":false,"name":"Time-Resolved Analysis Reveals Rapid Dynamics and Broad Scope of the CBP/p300 Acetylome.","description":"The acetyltransferases CBP and p300 are multifunctional transcriptional co-activators. Here, we combined quantitative proteomics with CBP/p300-specific catalytic inhibitors, bromodomain inhibitor, and gene knockout to reveal a comprehensive map of regulated acetylation sites and their dynamic turnover rates. CBP/p300 acetylates thousands of sites, including signature histone sites and a multitude of sites on signaling effectors and enhancer-associated transcriptional regulators. Time-resolved acetylome analyses identified a subset of CBP/p300-regulated sites with very rapid (<30 min) acetylation turnover, revealing a dynamic balance between acetylation and deacetylation. Quantification of acetylation, mRNA, and protein abundance after CBP/p300 inhibition reveals a kinetically competent net","dates":{"release":"2018-01-01T00:00:00Z","publication":"2018 Jun","modification":"2026-05-04T13:27:17.963Z","creation":"2019-07-25T07:07:58Z"},"accession":"S-EPMC6078418","cross_references":{"pubmed":["29804834"],"doi":["10.1016/j.cell.2018.04.033"]}}