biostudies-literatureSimonin MNational Science Foundation1769https://www.ebi.ac.uk/biostudies/studies/S-EPMC6079317biostudies-literatureUnknown9The environmental fate and potential impacts of nanopesticides on agroecosystems under realistic agricultural conditions are poorly understood. As a result, the benefits and risks of these novel formulations compared to the conventional products are currently unclear. Here, we examined the effects of repeated realistic exposures of the Cu(OH)₂ nanopesticide, Kocide 3000, on simulated agricultural pastureland in an outdoor mesocosm experiment over 1 year. The Kocide applications were performed alongside three different mineral fertilization levels (Ambient, Low, and High) to assess the environmental impacts of this nanopesticide under low-input or conventional farming scenarios. The effects of Kocide over time were monitored on forage biomass, plant mineral nutrient content, planFrontiers in microbiologyPlant and Microbial Responses to Repeated Cu(OH)₂ Nanopesticide Exposures Under Different Fertilization Levels in an Agro-Ecosystem.PMC6079317DBI-12662523331939EF-0830093Bergemann CMColman BPSimonin MRocca JDUnrine JMJudy JDCassar NAnderson SMTang WBernhardt ESfalsePlant and Microbial Responses to Repeated Cu(OH)₂ Nanopesticide Exposures Under Different Fertilization Levels in an Agro-Ecosystem.The environmental fate and potential impacts of nanopesticides on agroecosystems under realistic agricultural conditions are poorly understood. As a result, the benefits and risks of these novel formulations compared to the conventional products are currently unclear. Here, we examined the effects of repeated realistic exposures of the Cu(OH)₂ nanopesticide, Kocide 3000, on simulated agricultural pastureland in an outdoor mesocosm experiment over 1 year. The Kocide applications were performed alongside three different mineral fertilization levels (Ambient, Low, and High) to assess the environmental impacts of this nanopesticide under low-input or conventional farming scenarios. The effects of Kocide over time were monitored on forage biomass, plant mineral nutrient content, plan2018-01-01T00:00:00Z20182025-04-22T00:48:07.661Z2019-03-26T23:51:17ZS-EPMC60793173010858010.3389/fmicb.2018.01769