{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["41(2)"],"submitter":["Rosario Pinheiro DD"],"pubmed_abstract":["Gastric cancer (GC) remains one of the main causes of cancer-related death worldwide. There are two distinct histological types of GC: diffuse and intestinal. The latter is characterized by the presence of pre-neoplastic lesions. One of the most frequently altered enzymes in intestinal GC is COX-2, an important lesion marker. This work aimed to study COX-2 methylation and expression in N-methyl-N-Nitrosurea (MNU)-induced intestinal GC in six Sapajus apella animals. The partial promoter sequence of S. apella COX-2 gene was obtained and used to identify transcription factors and cis-regulatory element binding sites. The COX-2 methylation pattern was assessed using Methylation-Specific PCR (MSP), and expression was analyzed by immunohistochemistry (IHQ). A total of 20 samples were obtained. A"],"journal":["Genetics and molecular biology"],"pagination":["496-501"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6082229"],"repository":["biostudies-literature"],"pubmed_title":["COX-2 gene expression and methylation profile in Sapajus apella as an experimental model for gastric adenocarcinoma."],"pmcid":["PMC6082229"],"pubmed_authors":["Harada ML","Rosario Pinheiro DD","Rodriguez Burbano RM","Nascimento Borges BD"],"additional_accession":[]},"is_claimable":false,"name":"COX-2 gene expression and methylation profile in Sapajus apella as an experimental model for gastric adenocarcinoma.","description":"Gastric cancer (GC) remains one of the main causes of cancer-related death worldwide. There are two distinct histological types of GC: diffuse and intestinal. The latter is characterized by the presence of pre-neoplastic lesions. One of the most frequently altered enzymes in intestinal GC is COX-2, an important lesion marker. This work aimed to study COX-2 methylation and expression in N-methyl-N-Nitrosurea (MNU)-induced intestinal GC in six Sapajus apella animals. The partial promoter sequence of S. apella COX-2 gene was obtained and used to identify transcription factors and cis-regulatory element binding sites. The COX-2 methylation pattern was assessed using Methylation-Specific PCR (MSP), and expression was analyzed by immunohistochemistry (IHQ). A total of 20 samples were obtained. A","dates":{"release":"2018-01-01T00:00:00Z","publication":"2018 Apr./Jun","modification":"2025-04-21T17:36:41.442Z","creation":"2019-03-26T23:51:51Z"},"accession":"S-EPMC6082229","cross_references":{"pubmed":["29767663"],"doi":["10.1590/1678-4685-GMB-2016-0329"]}}