biostudies-literatureUnknown41(2)Rosario Pinheiro DDGastric cancer (GC) remains one of the main causes of cancer-related death worldwide. There are two distinct histological types of GC: diffuse and intestinal. The latter is characterized by the presence of pre-neoplastic lesions. One of the most frequently altered enzymes in intestinal GC is COX-2, an important lesion marker. This work aimed to study COX-2 methylation and expression in N-methyl-N-Nitrosurea (MNU)-induced intestinal GC in six Sapajus apella animals. The partial promoter sequence of S. apella COX-2 gene was obtained and used to identify transcription factors and cis-regulatory element binding sites. The COX-2 methylation pattern was assessed using Methylation-Specific PCR (MSP), and expression was analyzed by immunohistochemistry (IHQ). A total of 20 samples were obtained. AGenetics and molecular biology496-501https://www.ebi.ac.uk/biostudies/studies/S-EPMC6082229biostudies-literatureCOX-2 gene expression and methylation profile in Sapajus apella as an experimental model for gastric adenocarcinoma.PMC6082229Harada MLRosario Pinheiro DDRodriguez Burbano RMNascimento Borges BDfalseCOX-2 gene expression and methylation profile in Sapajus apella as an experimental model for gastric adenocarcinoma.Gastric cancer (GC) remains one of the main causes of cancer-related death worldwide. There are two distinct histological types of GC: diffuse and intestinal. The latter is characterized by the presence of pre-neoplastic lesions. One of the most frequently altered enzymes in intestinal GC is COX-2, an important lesion marker. This work aimed to study COX-2 methylation and expression in N-methyl-N-Nitrosurea (MNU)-induced intestinal GC in six Sapajus apella animals. The partial promoter sequence of S. apella COX-2 gene was obtained and used to identify transcription factors and cis-regulatory element binding sites. The COX-2 methylation pattern was assessed using Methylation-Specific PCR (MSP), and expression was analyzed by immunohistochemistry (IHQ). A total of 20 samples were obtained. A2018-01-01T00:00:00Z2018 Apr./Jun2025-04-21T17:36:41.442Z2019-03-26T23:51:51ZS-EPMC60822292976766310.1590/1678-4685-GMB-2016-0329