biostudies-literatureUnknown8(1)Lin ZNitrous oxide (N₂O) is formed during wastewater nitrogen removal processes. It is a strong greenhouse gas, however, if properly captured it can also be used as a renewable energy source. In this study, a nosZ-deficient strain of Pseudomonas aeruginosa was constructed. During growth under denitrifying conditions, the nosZ-deficient strain was more highly transcribing other genes from the denitrification pathway (narG, nirS, and norB) than the wild-type strain. This strain could also convert 85% of NO₂^--N to N₂O when it was grown with acetate compared to <0.6% by the wild-type strain. When a bioreactor treating synthetic wastewater with high NO₂^--N concentrations (700 mg/L) was inoculated with this strain, the N₂O cScientific reports11916https://www.ebi.ac.uk/biostudies/studies/S-EPMC6085377biostudies-literatureSignificant enhancement of nitrous oxide energy yields from wastewater achieved by bioaugmentation with a recombinant strain of Pseudomonas aeruginosa.PMC6085377Sun DLin ZDang YHolmes DEfalseSignificant enhancement of nitrous oxide energy yields from wastewater achieved by bioaugmentation with a recombinant strain of Pseudomonas aeruginosa.Nitrous oxide (N₂O) is formed during wastewater nitrogen removal processes. It is a strong greenhouse gas, however, if properly captured it can also be used as a renewable energy source. In this study, a nosZ-deficient strain of Pseudomonas aeruginosa was constructed. During growth under denitrifying conditions, the nosZ-deficient strain was more highly transcribing other genes from the denitrification pathway (narG, nirS, and norB) than the wild-type strain. This strain could also convert 85% of NO₂^--N to N₂O when it was grown with acetate compared to <0.6% by the wild-type strain. When a bioreactor treating synthetic wastewater with high NO₂^--N concentrations (700 mg/L) was inoculated with this strain, the N₂O c2018-01-01T00:00:00Z2018 Aug2025-04-04T08:43:33.453Z2019-03-26T23:51:19ZS-EPMC60853773009370610.1038/s41598-018-30326-8