biostudies-literaturePandya NJEuropean Research CouncilDutch Research Council (NWO)Marie Curie1218-1230https://www.ebi.ac.uk/biostudies/studies/S-EPMC6088136biostudies-literatureUnknown24(5)Lateral diffusion on the neuronal plasma membrane of the AMPA-type glutamate receptor (AMPAR) serves an important role in synaptic plasticity. We investigated the role of the secreted glycoprotein Noelin1 (Olfactomedin-1 or Pancortin) in AMPAR lateral mobility and its dependence on the extracellular matrix (ECM). We found that Noelin1 interacts with the AMPAR with high affinity, however, without affecting rise- and decay time and desensitization properties. Noelin1 co-localizes with synaptic and extra-synaptic AMPARs and is expressed at synapses in an activity-dependent manner. Single-particle tracking shows that Noelin1 reduces lateral mobility of both synaptic and extra-synaptic GluA1-containing receptors and affects short-term plasticity. While the ECM does not constrain the synaptic poCell reportsNoelin1 Affects Lateral Mobility of Synaptic AMPA Receptors.PMC6088136016.150.673281443MEST-ITN-2Lodder JCHeine MMansvelder HDFrischknecht RGonzalez-Lozano MABabai NSeeger CLi KWPandya NJGouwenberg YMack VSpijker SDanielson UHSmit ABfalseNoelin1 Affects Lateral Mobility of Synaptic AMPA Receptors.Lateral diffusion on the neuronal plasma membrane of the AMPA-type glutamate receptor (AMPAR) serves an important role in synaptic plasticity. We investigated the role of the secreted glycoprotein Noelin1 (Olfactomedin-1 or Pancortin) in AMPAR lateral mobility and its dependence on the extracellular matrix (ECM). We found that Noelin1 interacts with the AMPAR with high affinity, however, without affecting rise- and decay time and desensitization properties. Noelin1 co-localizes with synaptic and extra-synaptic AMPARs and is expressed at synapses in an activity-dependent manner. Single-particle tracking shows that Noelin1 reduces lateral mobility of both synaptic and extra-synaptic GluA1-containing receptors and affects short-term plasticity. While the ECM does not constrain the synaptic po2018-01-01T00:00:00Z2018 Jul2026-05-02T08:56:54.158Z2025-05-18T12:18:48.206ZS-EPMC60881363006797710.1016/j.celrep.2018.06.102