biostudies-literatureSpejo ABConselho Nacional de Desenvolvimento Científico e TecnológicoFundação de Amparo à Pesquisa do Estado de São Paulo230https://www.ebi.ac.uk/biostudies/studies/S-EPMC6092804biostudies-literatureUnknown15(1)<h4>Background</h4>Treatment of spinal cord injury is dependent on neuronal survival, appropriate synaptic circuit preservation, and inflammatory environment management. In this sense, mesenchymal stem cell (MSC) therapy is a promising tool that can reduce glial reaction and provide trophic factors to lesioned neurons.<h4>Methods</h4>Lewis adult female rats were submitted to a unilateral ventral funiculus cut at the spinal levels L4, L5, and L6. The animals were divided into the following groups: IA (intramedullary axotomy), IA + DMEM (Dulbecco's modified Eagle's medium), IA + FS (fibrin sealant), IA + MSC (10⁶ cells), and IA + FS + MSC (10⁶ cells). Seven days after injury, qPCR (n = 5) was performed to assess gene expression of VEGF, BDNF, iNOS2, arginase-1, TNF-α, IJournal of neuroinflammationNeuroprotection and immunomodulation following intraspinal axotomy of motoneurons by treatment with adult mesenchymal stem cells.PMC6092804303085/2017-72014/06892-3300553/2013-92013/16168-82012/22750-9Gomes DAChiarotto GBBarraviera BFerreira RSSpejo ABFerreira ADFOliveira ALRfalseNeuroprotection and immunomodulation following intraspinal axotomy of motoneurons by treatment with adult mesenchymal stem cells.<h4>Background</h4>Treatment of spinal cord injury is dependent on neuronal survival, appropriate synaptic circuit preservation, and inflammatory environment management. In this sense, mesenchymal stem cell (MSC) therapy is a promising tool that can reduce glial reaction and provide trophic factors to lesioned neurons.<h4>Methods</h4>Lewis adult female rats were submitted to a unilateral ventral funiculus cut at the spinal levels L4, L5, and L6. The animals were divided into the following groups: IA (intramedullary axotomy), IA + DMEM (Dulbecco's modified Eagle's medium), IA + FS (fibrin sealant), IA + MSC (10⁶ cells), and IA + FS + MSC (10⁶ cells). Seven days after injury, qPCR (n = 5) was performed to assess gene expression of VEGF, BDNF, iNOS2, arginase-1, TNF-α, I2018-01-01T00:00:00Z2018 Aug2025-04-19T10:56:58.101Z2019-03-26T23:51:43ZS-EPMC60928043010784810.1186/s12974-018-1268-4