{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Richter J"],"funding":["Austrian Science Fund FWF"],"pagination":["12182"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6093868"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["8(1)"],"pubmed_abstract":["Resolving functions of closely linked genes is challenging or nearly impossible with classical genetic tools. Four members of the Catharanthus roseus receptor-like kinase 1-like (CrRLK1L) family are clustered on Arabidopsis chromosome five. To resolve the potentially redundant functions of this subclass of CrRLK1Ls named MEDOS1 to 4 (MDS1 to 4), we generated a single CRISPR/Cas9 transformation vector using a Golden Gate based cloning system to target all four genes simultaneously. We introduce single mutations within and deletions between MDS genes as well as knock-outs of the whole 11 kb gene cluster. The large MDS cluster deletion was inherited in up to 25% of plants lacking the CRISPR/Cas9 construct in the T2 generation. In contrast to described phenotypes of already characterized CrRLK"],"journal":["Scientific reports"],"pubmed_title":["Multiplex mutagenesis of four clustered CrRLK1L with CRISPR/Cas9 exposes their growth regulatory roles in response to metal ions."],"pmcid":["PMC6093868"],"funding_grant_id":["I 1725-B16","I 1725"],"pubmed_authors":["Berger M","Richter J","Borowska M","Schoft V","Neuhold J","Hauser MT","Watson JM","Stasnik P","Stolt-Bergner P"],"additional_accession":[]},"is_claimable":false,"name":"Multiplex mutagenesis of four clustered CrRLK1L with CRISPR/Cas9 exposes their growth regulatory roles in response to metal ions.","description":"Resolving functions of closely linked genes is challenging or nearly impossible with classical genetic tools. Four members of the Catharanthus roseus receptor-like kinase 1-like (CrRLK1L) family are clustered on Arabidopsis chromosome five. To resolve the potentially redundant functions of this subclass of CrRLK1Ls named MEDOS1 to 4 (MDS1 to 4), we generated a single CRISPR/Cas9 transformation vector using a Golden Gate based cloning system to target all four genes simultaneously. We introduce single mutations within and deletions between MDS genes as well as knock-outs of the whole 11 kb gene cluster. The large MDS cluster deletion was inherited in up to 25% of plants lacking the CRISPR/Cas9 construct in the T2 generation. In contrast to described phenotypes of already characterized CrRLK","dates":{"release":"2018-01-01T00:00:00Z","publication":"2018 Aug","modification":"2025-04-19T10:58:12.78Z","creation":"2019-03-26T23:51:44Z"},"accession":"S-EPMC6093868","cross_references":{"pubmed":["30111865"],"doi":["10.1038/s41598-018-30711-3"]}}