biostudies-literatureBeach TGNational Institute of Neurological Disorders and StrokeNational Brain and Tissue Resource for Parkinson’s Disease and Related DisordersNIA NIH HHSArizona Alzheimer’s Disease Core CenterNINDS NIH HHSNational Institute on AgingMichael J. Fox Foundation for Parkinson’s Research793-802https://www.ebi.ac.uk/biostudies/studies/S-EPMC6097838biostudies-literatureUnknown77(9)Immunohistochemical (IHC) α-synuclein (Asyn) pathology in peripheral biopsies may be a biomarker of Parkinson disease (PD). The multi-center Systemic Synuclein Sampling Study (S4) is evaluating IHC Asyn pathology within skin, colon and submandibular gland biopsies from 60 PD and 20 control subjects. Asyn pathology is being evaluated by a blinded panel of specially trained neuropathologists. Preliminary work assessed 2 candidate immunoperoxidase methods using a set of PD and control autopsy-derived sections from formalin-fixed, paraffin-embedded blocks of the 3 tissues. Both methods had 100% specificity; one, utilizing the 5C12 monoclonal antibody, was more sensitive in skin (67% vs 33%), and was chosen for further use in S4. Four trainee neuropathologists were trained to perform S4 histopaJournal of neuropathology and experimental neurologyImmunohistochemical Method and Histopathology Judging for the Systemic Synuclein Sampling Study (S4).PMC6097838U24 NS072026P30 AG019610P30 AG19610Purri RJennings DNoyes-Lloyd CCoffey CSDziadek SMunoz DGLinder CDuffy AAkhtar RKremer TCorbille AGEcklund DGoßmann JAmara ARussell DLetournel FForoud TSeibyl JWhite CLKonig FChahine LRiss HDave KDMollenhauer BCrary JFMarras CGlass MJLott DWalker JEPotter MKeshavarzian AVisanji NRiley LCanamero MSue LIGuilmette LCase RSmith DCresswell MAdler CHRothberg BSerrano GEBlair CSchneider JArnedo VSystemic Synuclein Sampling Study (S4)Hogarth PDerkinderen PIntorcia AJKopil CMMitchell CBeach TGOza VSade HfalseImmunohistochemical Method and Histopathology Judging for the Systemic Synuclein Sampling Study (S4).Immunohistochemical (IHC) α-synuclein (Asyn) pathology in peripheral biopsies may be a biomarker of Parkinson disease (PD). The multi-center Systemic Synuclein Sampling Study (S4) is evaluating IHC Asyn pathology within skin, colon and submandibular gland biopsies from 60 PD and 20 control subjects. Asyn pathology is being evaluated by a blinded panel of specially trained neuropathologists. Preliminary work assessed 2 candidate immunoperoxidase methods using a set of PD and control autopsy-derived sections from formalin-fixed, paraffin-embedded blocks of the 3 tissues. Both methods had 100% specificity; one, utilizing the 5C12 monoclonal antibody, was more sensitive in skin (67% vs 33%), and was chosen for further use in S4. Four trainee neuropathologists were trained to perform S4 histopa2018-01-01T00:00:00Z2018 Sep2026-04-07T16:57:33.969Z2019-09-04T07:01:29ZS-EPMC60978383010760410.1093/jnen/nly056