biostudies-literatureZhang HNational Natural Science Foundation of ChinaNatural Science Foundation of TianjinE1656https://www.ebi.ac.uk/biostudies/studies/S-EPMC6099698biostudies-literatureUnknown23(7)Epimedium herb is one of the most vital traditional Chinese medicines (TCMs), which is used for “nourishing the kidney and reinforcing the Yang”. In the guidance of TCM theory, Epimedium herb is usually processed with lamb oil to increase its efficacy. The contents of active ingredients in different Epimedium are significantly varied, which may derive from their different species, regions and processing methods. In this research, 13 batches of raw Epimedium collected from 6 provinces were identified. After optimization of the processing method of Epimedium, a liquid chromatography⁻mass spectrometry (LC⁻MS/MS) method for simultaneous determination of 16 compounds was established to evaluate the quality of raw and processed. Then the multivariate statistical technique was applied to compare Molecules (Basel, Switzerland)Comparison of the Active Compositions between Raw and Processed <i>Epimedium</i> from Different Species.PMC609969817JCYBJC28900, 15JCYBJC2930081703702, 81673824, 81503457Sun MHao JOuyang HHe JDou ZZhang HWang HChen XWei JChang YfalseComparison of the Active Compositions between Raw and Processed <i>Epimedium</i> from Different Species.Epimedium herb is one of the most vital traditional Chinese medicines (TCMs), which is used for “nourishing the kidney and reinforcing the Yang”. In the guidance of TCM theory, Epimedium herb is usually processed with lamb oil to increase its efficacy. The contents of active ingredients in different Epimedium are significantly varied, which may derive from their different species, regions and processing methods. In this research, 13 batches of raw Epimedium collected from 6 provinces were identified. After optimization of the processing method of Epimedium, a liquid chromatography⁻mass spectrometry (LC⁻MS/MS) method for simultaneous determination of 16 compounds was established to evaluate the quality of raw and processed. Then the multivariate statistical technique was applied to compare 2018-01-01T00:00:00Z2018 Jul2025-04-05T14:47:28.732Z2019-03-27T00:06:39ZS-EPMC60996982998648610.3390/molecules23071656