{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Wang M"],"funding":["National Natural Science Foundation of China"],"pagination":["E1253"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6100196"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["23(6)"],"pubmed_abstract":["Take-all, caused by <i>Gaeumannomyces tritici</i>, is one of the most important wheat root diseases worldwide, as it results in serious yield losses. In this study, <i>G. tritici</i> was transformed to express the hygromycin B phosphotransferase using a combined protoplast and polyethylene glycol (PEG)-mediated transformation technique. Based on a series of single-factor experimental results, three major factors-temperature, enzyme lysis time, and concentration of the lysing enzyme-were selected as the independent variables, which were optimized using the response surface methodology. A higher protoplast yield of 9.83 × 10⁷ protoplasts/mL was observed, and the protoplast vitality was also high, reaching 96.27% after optimization. Protoplasts were isolated under the optimal conditions, with"],"journal":["Molecules (Basel, Switzerland)"],"pubmed_title":["Optimization of Production Conditions for Protoplasts and Polyethylene Glycol-Mediated Transformation of <i>Gaeumannomyces tritici</i>."],"pmcid":["PMC6100196"],"funding_grant_id":["31272074"],"pubmed_authors":["Wang M","Feng J","Zhang J","Han L","Zhang X","Wang L"],"additional_accession":[]},"is_claimable":false,"name":"Optimization of Production Conditions for Protoplasts and Polyethylene Glycol-Mediated Transformation of <i>Gaeumannomyces tritici</i>.","description":"Take-all, caused by <i>Gaeumannomyces tritici</i>, is one of the most important wheat root diseases worldwide, as it results in serious yield losses. In this study, <i>G. tritici</i> was transformed to express the hygromycin B phosphotransferase using a combined protoplast and polyethylene glycol (PEG)-mediated transformation technique. Based on a series of single-factor experimental results, three major factors-temperature, enzyme lysis time, and concentration of the lysing enzyme-were selected as the independent variables, which were optimized using the response surface methodology. A higher protoplast yield of 9.83 × 10⁷ protoplasts/mL was observed, and the protoplast vitality was also high, reaching 96.27% after optimization. Protoplasts were isolated under the optimal conditions, with","dates":{"release":"2018-01-01T00:00:00Z","publication":"2018 May","modification":"2025-04-05T14:47:32.172Z","creation":"2019-03-27T00:06:40Z"},"accession":"S-EPMC6100196","cross_references":{"pubmed":["29794975"],"doi":["10.3390/molecules23061253"]}}