biostudies-literatureUnknown10Belykh E<h4>Background</h4>Confocal laser endomicroscopy (CLE) is used during fluorescence-guided brain tumor surgery for intraoperative microscopy of tumor tissue with cellular resolution. CLE could augment and expedite intraoperative decision-making and potentially aid in diagnosis and removal of tumor tissue.<h4>Objective</h4>To describe an extension of CLE imaging modality that produces Z-stack images and three-dimensional (3D) pseudocolored volumetric images.<h4>Materials and methods</h4>Hand-held probe-based CLE was used to collect images from GL261-luc2 gliomas in C57BL/6 mice and from human brain tumor biopsies. The mice were injected with fluorescein sodium (FNa) before imaging. Patients received FNa intraoperatively, and biopsies were imaged immediately in the operating room. Some specimCancer management and research3109-3123https://www.ebi.ac.uk/biostudies/studies/S-EPMC6124793biostudies-literatureProbe-based three-dimensional confocal laser endomicroscopy of brain tumors: technical note.PMC6124793Eschbacher JMBozkurt BScheck ACYagmurlu KWoolf ECBelykh EPatel AAMiller EJPreul MCNakaji PfalseProbe-based three-dimensional confocal laser endomicroscopy of brain tumors: technical note.<h4>Background</h4>Confocal laser endomicroscopy (CLE) is used during fluorescence-guided brain tumor surgery for intraoperative microscopy of tumor tissue with cellular resolution. CLE could augment and expedite intraoperative decision-making and potentially aid in diagnosis and removal of tumor tissue.<h4>Objective</h4>To describe an extension of CLE imaging modality that produces Z-stack images and three-dimensional (3D) pseudocolored volumetric images.<h4>Materials and methods</h4>Hand-held probe-based CLE was used to collect images from GL261-luc2 gliomas in C57BL/6 mice and from human brain tumor biopsies. The mice were injected with fluorescein sodium (FNa) before imaging. Patients received FNa intraoperatively, and biopsies were imaged immediately in the operating room. Some specim2018-01-01T00:00:00Z20182026-06-10T03:19:26.6Z2026-06-10T03:12:00.876ZS-EPMC61247933021430410.2147/CMAR.S165980