biostudies-literatureBostanci NNational Institute of Allergy and Infectious DiseasesNIDCR NIH HHSFP7 HealthNIAID NIH HHSNational Institute of Dental and Craniofacial Research3153-3175https://www.ebi.ac.uk/biostudies/studies/S-EPMC6128774biostudies-literatureUnknown17(9)Periodontitis is a prevalent chronic inflammatory disease associated with dysbiosis. Although complement inhibition has been successfully used to treat periodontitis in animal models, studies globally analyzing inflamed tissue proteins to glean insight into possible mechanisms of action are missing. Using quantitative shotgun proteomics, we aimed to investigate differences in composition of inflammatory gingival tissue exudate ("gingival crevicular fluid"; GCF), before and after local administration of an inhibitor of the central complement component, C3, in nonhuman primates. The C3 inhibitor, Cp40 (also known as AMY-101) was administered locally in the maxillary gingival tissue of cynomolgus monkeys with established periodontitis, either once a week (1×-treatment; n = 5 animals) or threeJournal of proteome researchGingival Exudatome Dynamics Implicate Inhibition of the Alternative Complement Pathway in the Protective Action of the C3 Inhibitor Cp40 in Nonhuman Primate Periodontitis.PMC6128774DE024153DE024716AI068730P01 AI068730R01 DE024716R01 DE015254602699R01 DE024153DE015254Garcia CAGMaekawa TReis ESLambris JDTuplano JVGrossmann JPanse CLi XResuello RRGBao KBostanci NBriones RAHajishengallis GfalseGingival Exudatome Dynamics Implicate Inhibition of the Alternative Complement Pathway in the Protective Action of the C3 Inhibitor Cp40 in Nonhuman Primate Periodontitis.Periodontitis is a prevalent chronic inflammatory disease associated with dysbiosis. Although complement inhibition has been successfully used to treat periodontitis in animal models, studies globally analyzing inflamed tissue proteins to glean insight into possible mechanisms of action are missing. Using quantitative shotgun proteomics, we aimed to investigate differences in composition of inflammatory gingival tissue exudate ("gingival crevicular fluid"; GCF), before and after local administration of an inhibitor of the central complement component, C3, in nonhuman primates. The C3 inhibitor, Cp40 (also known as AMY-101) was administered locally in the maxillary gingival tissue of cynomolgus monkeys with established periodontitis, either once a week (1×-treatment; n = 5 animals) or three2018-01-01T00:00:00Z2018 Sep2026-04-08T01:40:58.389Z2019-09-12T07:00:33ZS-EPMC61287743011111210.1021/acs.jproteome.8b00263