{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["9"],"submitter":["Khan M"],"pubmed_abstract":["Early blight (EB), caused by the pathogen <i>Alternaria solani</i>, is a major threat to global potato and tomato production. Early and accurate diagnosis of this disease is therefore important. In this study, we conducted a loop-mediated isothermal amplification (LAMP) assay, as well as conventional polymerase chain reaction (PCR), nested PCR, and quantitative real-time PCR (RT-qPCR) assays to determine which of these techniques was less time consuming, more sensitive, and more accurate. We based our assays on sequence-characterized amplified regions of the <i>histidine kinase</i> gene with an accession number (FJ424058). The LAMP assay provided more rapid and accurate results, amplifying the target pathogen in less than 60 min at 63°C, with 10-fold greater sensitivity than conventional P"],"journal":["Frontiers in microbiology"],"pagination":["2089"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6129767"],"repository":["biostudies-literature"],"pubmed_title":["Comparative Evaluation of the LAMP Assay and PCR-Based Assays for the Rapid Detection of <i>Alternaria solani</i>."],"pmcid":["PMC6129767"],"pubmed_authors":["Weng Q","Chen Q","Khan M","Wang R","Liu P","Li B"],"additional_accession":[]},"is_claimable":false,"name":"Comparative Evaluation of the LAMP Assay and PCR-Based Assays for the Rapid Detection of <i>Alternaria solani</i>.","description":"Early blight (EB), caused by the pathogen <i>Alternaria solani</i>, is a major threat to global potato and tomato production. Early and accurate diagnosis of this disease is therefore important. In this study, we conducted a loop-mediated isothermal amplification (LAMP) assay, as well as conventional polymerase chain reaction (PCR), nested PCR, and quantitative real-time PCR (RT-qPCR) assays to determine which of these techniques was less time consuming, more sensitive, and more accurate. We based our assays on sequence-characterized amplified regions of the <i>histidine kinase</i> gene with an accession number (FJ424058). The LAMP assay provided more rapid and accurate results, amplifying the target pathogen in less than 60 min at 63°C, with 10-fold greater sensitivity than conventional P","dates":{"release":"2018-01-01T00:00:00Z","publication":"2018","modification":"2026-04-08T01:45:02.349Z","creation":"2019-03-26T23:56:35Z"},"accession":"S-EPMC6129767","cross_references":{"pubmed":["30233554"],"doi":["10.3389/fmicb.2018.02089"]}}