biostudies-literatureUnknown9Khan MEarly blight (EB), caused by the pathogen <i>Alternaria solani</i>, is a major threat to global potato and tomato production. Early and accurate diagnosis of this disease is therefore important. In this study, we conducted a loop-mediated isothermal amplification (LAMP) assay, as well as conventional polymerase chain reaction (PCR), nested PCR, and quantitative real-time PCR (RT-qPCR) assays to determine which of these techniques was less time consuming, more sensitive, and more accurate. We based our assays on sequence-characterized amplified regions of the <i>histidine kinase</i> gene with an accession number (FJ424058). The LAMP assay provided more rapid and accurate results, amplifying the target pathogen in less than 60 min at 63°C, with 10-fold greater sensitivity than conventional PFrontiers in microbiology2089https://www.ebi.ac.uk/biostudies/studies/S-EPMC6129767biostudies-literatureComparative Evaluation of the LAMP Assay and PCR-Based Assays for the Rapid Detection of <i>Alternaria solani</i>.PMC6129767Weng QChen QKhan MWang RLiu PLi BfalseComparative Evaluation of the LAMP Assay and PCR-Based Assays for the Rapid Detection of <i>Alternaria solani</i>.Early blight (EB), caused by the pathogen <i>Alternaria solani</i>, is a major threat to global potato and tomato production. Early and accurate diagnosis of this disease is therefore important. In this study, we conducted a loop-mediated isothermal amplification (LAMP) assay, as well as conventional polymerase chain reaction (PCR), nested PCR, and quantitative real-time PCR (RT-qPCR) assays to determine which of these techniques was less time consuming, more sensitive, and more accurate. We based our assays on sequence-characterized amplified regions of the <i>histidine kinase</i> gene with an accession number (FJ424058). The LAMP assay provided more rapid and accurate results, amplifying the target pathogen in less than 60 min at 63°C, with 10-fold greater sensitivity than conventional P2018-01-01T00:00:00Z20182026-04-08T01:45:02.349Z2019-03-26T23:56:35ZS-EPMC61297673023355410.3389/fmicb.2018.02089