biostudies-literatureLi MAnhui Major Demonstration Project for Leading Talent Team on Tea Chemistry and HealthNatural Science Foundation of Anhui ProvinceNatural Science Foundation of Shandong ProvinceNational Natural Science Foundation of China4276-4286https://www.ebi.ac.uk/biostudies/studies/S-EPMC6133834biostudies-literatureUnknown55(10)We describe a novel analytical method for quantification of free amino acids in tea using variable mobile phase pH, elution gradient and column temperature of reversed-phase high-performance liquid chromatography (RP-HPLC). The study of mobile phase pH 5.7 was chosen to simultaneous quantification of 19 free amino acids in tea, while it improved maximum resolution of glutamine, histidine and theanine. Elution gradient was adapted for enhancing the solution of free amino acids, mainly because of adjustment of mobile phase A and B. The column temperature of 40 °C was conducive to separate free amino acids in tea. The limit of detection (LOD) and limit of quantitation (LOQ) of this method were in the range of 0.097-0.228 nmol/mL and 0.323-0.761 nmol/mL, respectively. The relative standard devJournal of food science and technologyDetermination of free amino acids in tea by a novel method of reversed-phase high performance liquid chromatography applying 6-Aminoquinolyl-<i>N</i>-Hydroxysuccinimidyl carbamate reagent.PMC61338341508085MC5931170283ZR2014CP010Gu CHo CTGu XLi DHajano JUDWei CTai YNing JWan XLi MSong YJiao WfalseDetermination of free amino acids in tea by a novel method of reversed-phase high performance liquid chromatography applying 6-Aminoquinolyl-<i>N</i>-Hydroxysuccinimidyl carbamate reagent.We describe a novel analytical method for quantification of free amino acids in tea using variable mobile phase pH, elution gradient and column temperature of reversed-phase high-performance liquid chromatography (RP-HPLC). The study of mobile phase pH 5.7 was chosen to simultaneous quantification of 19 free amino acids in tea, while it improved maximum resolution of glutamine, histidine and theanine. Elution gradient was adapted for enhancing the solution of free amino acids, mainly because of adjustment of mobile phase A and B. The column temperature of 40 °C was conducive to separate free amino acids in tea. The limit of detection (LOD) and limit of quantitation (LOQ) of this method were in the range of 0.097-0.228 nmol/mL and 0.323-0.761 nmol/mL, respectively. The relative standard dev2018-01-01T00:00:00Z2018 Oct2025-04-21T22:04:48.13Z2019-10-11T07:16:03ZS-EPMC61338343022842610.1007/s13197-018-3366-9