{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Daniszewski M"],"funding":["Ophthalmic Research Institute of Australia","Philip Neal bequest","Joan and Peter Clemenger Foundation","Retina Australia","Victorian Government","University of Melbourne","National Health and Medical Research Council","Australian Research Council"],"pagination":["30-39"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6135898"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["7"],"pubmed_abstract":["We assessed the pluripotency of human induced pluripotent stem cells (iPSCs) maintained on an automated platform using StemFlex and TeSR-E8 media. Analysis of transcriptome of single cells revealed similar expression of core pluripotency genes, as well as genes associated with naive and primed states of pluripotency. Analysis of individual cells from four samples consisting of two different iPSC lines each grown in the two culture media revealed a shared subpopulation structure with three main subpopulations different in pluripotency states. By implementing a machine learning approach, we estimated that most cells within each subpopulation are very similar between all four samples. The single-cell RNA sequencing analysis of iPSC lines grown in both media reports the molecular signature in StemFlex medium and how it compares to that observed in the TeSR-E8 medium."],"journal":["iScience"],"pubmed_title":["Single-Cell Profiling Identifies Key Pathways Expressed by iPSCs Cultured in Different Commercial Media."],"pmcid":["PMC6135898"],"funding_grant_id":["FT140100047"],"pubmed_authors":["Lidgerwood GE","Andersen SB","Mackey DA","Laslett AL","Hewitt AW","Liang HH","Powell JE","Nguyen Q","Sivakumaran P","Kulkarni T","Vickers JC","Chy HS","Chevalier S","Conquest A","Senabouth A","Daniszewski M","Rooney LA","Pebay A","Craig JE","Crombie DE","Hernandez D","Singh V"],"additional_accession":[]},"is_claimable":false,"name":"Single-Cell Profiling Identifies Key Pathways Expressed by iPSCs Cultured in Different Commercial Media.","description":"We assessed the pluripotency of human induced pluripotent stem cells (iPSCs) maintained on an automated platform using StemFlex and TeSR-E8 media. Analysis of transcriptome of single cells revealed similar expression of core pluripotency genes, as well as genes associated with naive and primed states of pluripotency. Analysis of individual cells from four samples consisting of two different iPSC lines each grown in the two culture media revealed a shared subpopulation structure with three main subpopulations different in pluripotency states. By implementing a machine learning approach, we estimated that most cells within each subpopulation are very similar between all four samples. The single-cell RNA sequencing analysis of iPSC lines grown in both media reports the molecular signature in StemFlex medium and how it compares to that observed in the TeSR-E8 medium.","dates":{"release":"2018-01-01T00:00:00Z","publication":"2018 Sep","modification":"2025-04-04T10:17:49.923Z","creation":"2019-03-26T23:58:17Z"},"accession":"S-EPMC6135898","cross_references":{"pubmed":["30267684"],"doi":["10.1016/j.isci.2018.08.016"]}}