biostudies-literaturePetyuk VANational Center for Research ResourcesNIA NIH HHSMedical Research CouncilNIMH NIH HHSNational Institute for Health Research (NIHR)NINDS NIH HHSNational Institutes of HealthNIHNational Institute of General Medical SciencesNIGMS NIH HHS2721-2739https://www.ebi.ac.uk/biostudies/studies/S-EPMC6136080biostudies-literatureUnknown141(9)Our hypothesis is that changes in gene and protein expression are crucial to the development of late-onset Alzheimer’s disease. Previously we examined how DNA alleles control downstream expression of RNA transcripts and how those relationships are changed in late-onset Alzheimer’s disease. We have now examined how proteins are incorporated into networks in two separate series and evaluated our outputs in two different cell lines. Our pipeline included the following steps: (i) predicting expression quantitative trait loci; (ii) determining differential expression; (iii) analysing networks of transcript and peptide relationships; and (iv) validating effects in two separate cell lines. We performed all our analysis in two separate brain series to validate effects. Our two series iBrain : a journal of neurologyThe human brainome: network analysis identifies HSPA2 as a novel Alzheimer’s disease target.PMC6136080P30 AG013846P50 AG005128P41GM103493P30 AG053760P50 AG005146AG041232P50 AG005681P50 AG005144G0502157P41 GM103493P30 AG019610P41RR018522G0900652R01 AG041232R01 AG034504P50 AG005134U01 AG016976P50 AG005136NF-SI-0611-10048P30 AG010161RF1 AG057457P50-AG08671RF1 AG015819P50 MH060451G1100540G0400074P50 NS039764R01 AG017917P50 AG016570Lieberman APBeckmann NDSmith RDAndreev VLovestone SMyers AJNavarro LXie FFerrer IPiehowski PDWang SSchadt EEngel ADe Jager PSue LIHardy JAMorris CMReiman EMHenrion MYRErvin JFChang RRamirez-Restrepo MMcKeith IGPerry RHSerrano GEHulette CMClarke JPetyuk VASchneider JABennett DAZhu KGuettoche TWoltjer RLBeach TGAlbin RLMash DCHuentelman MJfalseThe human brainome: network analysis identifies HSPA2 as a novel Alzheimer’s disease target.Our hypothesis is that changes in gene and protein expression are crucial to the development of late-onset Alzheimer’s disease. Previously we examined how DNA alleles control downstream expression of RNA transcripts and how those relationships are changed in late-onset Alzheimer’s disease. We have now examined how proteins are incorporated into networks in two separate series and evaluated our outputs in two different cell lines. Our pipeline included the following steps: (i) predicting expression quantitative trait loci; (ii) determining differential expression; (iii) analysing networks of transcript and peptide relationships; and (iv) validating effects in two separate cell lines. We performed all our analysis in two separate brain series to validate effects. Our two series i2018-01-01T00:00:00Z2018 Sep2025-04-18T13:29:14.2Z2019-03-26T23:56:53ZS-EPMC61360803013721210.1093/brain/awy215