{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Zhang J"],"funding":["CAMS Innovation Fund for Medical Sciences"],"pagination":["225"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6136228"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["37(1)"],"pubmed_abstract":["<h4>Background</h4>Tumor recurrence, the chief reason for poor prognosis of glioma, is largely attributed to glioma stem cells (GSCs) and epithelial-mesenchymal transition (EMT). However, the mechanisms among them remain unknown. Here, we determined whether leucine-rich repeat-containing G protein-coupled receptor 5 (LGR5), known as a stem cell marker for colon cancer and gastric cancer, can serve as a novel GSC marker involved in EMT and a therapeutic target in glioma.<h4>Methods</h4>Stemness properties were examined in FACS-isolated LGR5<sup>+</sup>/LGR5<sup>-</sup> cells. Reported stem cell markers, EMT and the Wnt/β-catenin pathway were examined in stable LGR5 knockdown or overexpressed GSCs by Western Blot. The treatment experiment was performed in an intracranial orthotopic xenograft"],"journal":["Journal of experimental & clinical cancer research : CR"],"pubmed_title":["LGR5, a novel functional glioma stem cell marker, promotes EMT by activating the Wnt/β-catenin pathway and predicts poor survival of glioma patients."],"pmcid":["PMC6136228"],"funding_grant_id":["Nos. 2016-I2M-3-013, 2017-I2M-3-005"],"pubmed_authors":["Sun L","Zhan P","Zhang F","Zhang J","Chen M","Ran Y","Wan J","Cai H"],"additional_accession":[]},"is_claimable":false,"name":"LGR5, a novel functional glioma stem cell marker, promotes EMT by activating the Wnt/β-catenin pathway and predicts poor survival of glioma patients.","description":"<h4>Background</h4>Tumor recurrence, the chief reason for poor prognosis of glioma, is largely attributed to glioma stem cells (GSCs) and epithelial-mesenchymal transition (EMT). However, the mechanisms among them remain unknown. Here, we determined whether leucine-rich repeat-containing G protein-coupled receptor 5 (LGR5), known as a stem cell marker for colon cancer and gastric cancer, can serve as a novel GSC marker involved in EMT and a therapeutic target in glioma.<h4>Methods</h4>Stemness properties were examined in FACS-isolated LGR5<sup>+</sup>/LGR5<sup>-</sup> cells. Reported stem cell markers, EMT and the Wnt/β-catenin pathway were examined in stable LGR5 knockdown or overexpressed GSCs by Western Blot. The treatment experiment was performed in an intracranial orthotopic xenograft","dates":{"release":"2018-01-01T00:00:00Z","publication":"2018 Sep","modification":"2026-06-13T04:01:09.653Z","creation":"2019-03-26T23:56:01Z"},"accession":"S-EPMC6136228","cross_references":{"pubmed":["30208924"],"doi":["10.1186/s13046-018-0864-6"]}}