biostudies-literatureZhang JCAMS Innovation Fund for Medical Sciences225https://www.ebi.ac.uk/biostudies/studies/S-EPMC6136228biostudies-literatureUnknown37(1)<h4>Background</h4>Tumor recurrence, the chief reason for poor prognosis of glioma, is largely attributed to glioma stem cells (GSCs) and epithelial-mesenchymal transition (EMT). However, the mechanisms among them remain unknown. Here, we determined whether leucine-rich repeat-containing G protein-coupled receptor 5 (LGR5), known as a stem cell marker for colon cancer and gastric cancer, can serve as a novel GSC marker involved in EMT and a therapeutic target in glioma.<h4>Methods</h4>Stemness properties were examined in FACS-isolated LGR5⁺/LGR5^- cells. Reported stem cell markers, EMT and the Wnt/β-catenin pathway were examined in stable LGR5 knockdown or overexpressed GSCs by Western Blot. The treatment experiment was performed in an intracranial orthotopic xenograftJournal of experimental & clinical cancer research : CRLGR5, a novel functional glioma stem cell marker, promotes EMT by activating the Wnt/β-catenin pathway and predicts poor survival of glioma patients.PMC6136228Nos. 2016-I2M-3-013, 2017-I2M-3-005Sun LZhan PZhang FZhang JChen MRan YWan JCai HfalseLGR5, a novel functional glioma stem cell marker, promotes EMT by activating the Wnt/β-catenin pathway and predicts poor survival of glioma patients.<h4>Background</h4>Tumor recurrence, the chief reason for poor prognosis of glioma, is largely attributed to glioma stem cells (GSCs) and epithelial-mesenchymal transition (EMT). However, the mechanisms among them remain unknown. Here, we determined whether leucine-rich repeat-containing G protein-coupled receptor 5 (LGR5), known as a stem cell marker for colon cancer and gastric cancer, can serve as a novel GSC marker involved in EMT and a therapeutic target in glioma.<h4>Methods</h4>Stemness properties were examined in FACS-isolated LGR5⁺/LGR5^- cells. Reported stem cell markers, EMT and the Wnt/β-catenin pathway were examined in stable LGR5 knockdown or overexpressed GSCs by Western Blot. The treatment experiment was performed in an intracranial orthotopic xenograft2018-01-01T00:00:00Z2018 Sep2026-06-13T04:01:09.653Z2019-03-26T23:56:01ZS-EPMC61362283020892410.1186/s13046-018-0864-6