{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["6"],"submitter":["Hajighasem A"],"pubmed_abstract":["<h4>Background</h4>Non-alcoholic fatty liver disease (NAFLD) is the most common chronic liver disorder. This study aims to consider effects of resveratrol, exercise and their combination on <i>Farnesoid X receptor (<i>Fxr</i>), the liver X receptor (<i>Lxr</i>) and Sirtuin 1 (Sirt 1)</i> genes expression in the liver of elderly rats with NAFLD.<h4>Methods</h4>Rats with NAFLD were randomly divided into seven groups including patient, saline, resveratrol (RSV), interval exercise, continuous exercise, interval exercise + RSV and continuous exercise + RSV. Levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP) in the liver tissue were measured using specific ELISA kits. A TUNEL assay kit was used for the assessment of hepatic cells apoptosis. Lipid profiles were considered by measuring the serum triglyceride, cholesterol, LDL, and HDL. Expression of <i>Sirt1</i>, <i>Lxr</i> and <i>Fxr</i> genes was considered using RT-PCR.<h4>Results</h4>Resveratrol administration alone or combined with exercise training significantly improved the expression of <i>Sirt1</i>, <i>Lxr</i> and <i>Fxr</i> genes (<i>p</i> < 0.05) in the hepatic tissue of rats with NAFLD, while levels of AST, ALT, ALP enzymes, as well as apoptotic cells were significantly decreased (<i>p</i> < 0.05).<h4>Discussion</h4>Although resveratrol alone improves the expression of <i>Sirt1</i>, <i>Lxr</i> and <i>Fxr</i>, as well as liver function, combined therapy with exercise training is more effective to improve NAFLD."],"journal":["PeerJ"],"pagination":["e5522"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6136396"],"repository":["biostudies-literature"],"pubmed_title":["Effects of resveratrol, exercises and their combination on <i>Farnesoid X receptor, Liver X receptor and Sirtuin 1</i> gene expression and apoptosis in the liver of elderly rats with nonalcoholic fatty liver."],"pmcid":["PMC6136396"],"pubmed_authors":["Farzanegi P","Mazaheri Z","Naghizadeh M","Salehi G","Hajighasem A"],"additional_accession":[]},"is_claimable":false,"name":"Effects of resveratrol, exercises and their combination on <i>Farnesoid X receptor, Liver X receptor and Sirtuin 1</i> gene expression and apoptosis in the liver of elderly rats with nonalcoholic fatty liver.","description":"<h4>Background</h4>Non-alcoholic fatty liver disease (NAFLD) is the most common chronic liver disorder. This study aims to consider effects of resveratrol, exercise and their combination on <i>Farnesoid X receptor (<i>Fxr</i>), the liver X receptor (<i>Lxr</i>) and Sirtuin 1 (Sirt 1)</i> genes expression in the liver of elderly rats with NAFLD.<h4>Methods</h4>Rats with NAFLD were randomly divided into seven groups including patient, saline, resveratrol (RSV), interval exercise, continuous exercise, interval exercise + RSV and continuous exercise + RSV. Levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP) in the liver tissue were measured using specific ELISA kits. A TUNEL assay kit was used for the assessment of hepatic cells apoptosis. Lipid profiles were considered by measuring the serum triglyceride, cholesterol, LDL, and HDL. Expression of <i>Sirt1</i>, <i>Lxr</i> and <i>Fxr</i> genes was considered using RT-PCR.<h4>Results</h4>Resveratrol administration alone or combined with exercise training significantly improved the expression of <i>Sirt1</i>, <i>Lxr</i> and <i>Fxr</i> genes (<i>p</i> < 0.05) in the hepatic tissue of rats with NAFLD, while levels of AST, ALT, ALP enzymes, as well as apoptotic cells were significantly decreased (<i>p</i> < 0.05).<h4>Discussion</h4>Although resveratrol alone improves the expression of <i>Sirt1</i>, <i>Lxr</i> and <i>Fxr</i>, as well as liver function, combined therapy with exercise training is more effective to improve NAFLD.","dates":{"release":"2018-01-01T00:00:00Z","publication":"2018","modification":"2021-02-21T03:58:42Z","creation":"2019-03-26T23:56:24Z"},"accession":"S-EPMC6136396","cross_references":{"pubmed":["30221089"],"doi":["10.7717/peerj.5522"]}}