biostudies-literatureZheng QQNational Natural Science Foundation of China726-734https://www.ebi.ac.uk/biostudies/studies/S-EPMC6137421biostudies-literatureUnknown19(9)p-Nitrophenylphosphate (PNPP) is usually employed as the substrate for enzyme-linked immunosorbent assays. p-Nitrophenol (PNP), the product of PNPP, with the catalyst alkaline phosphatase (ALP), will passivate an electrode, which limits applications in electrochemical analysis. A novel anti-passivation ink used in the preparation of a graphene/ionic liquid/chitosan composited (rGO/IL/Chi) electrode is proposed to solve the problem. The anti-passivation electrode was fabricated by directly writing the graphene-ionic liquid-chitosan composite on a single-side conductive gold strip. A glassy carbon electrode, a screen-printed electrode, and a graphene-chitosan composite-modified screen-printed electrode were investigated for comparison. Scanning electron microscopy was used to characterize thJournal of Zhejiang University. Science. BAn anti-passivation ink for the preparation of electrodes for use in electrochemical immunoassays.PMC613742131571918Ye ZZWu JYing YBLu YCPing JFZheng QQfalseAn anti-passivation ink for the preparation of electrodes for use in electrochemical immunoassays.p-Nitrophenylphosphate (PNPP) is usually employed as the substrate for enzyme-linked immunosorbent assays. p-Nitrophenol (PNP), the product of PNPP, with the catalyst alkaline phosphatase (ALP), will passivate an electrode, which limits applications in electrochemical analysis. A novel anti-passivation ink used in the preparation of a graphene/ionic liquid/chitosan composited (rGO/IL/Chi) electrode is proposed to solve the problem. The anti-passivation electrode was fabricated by directly writing the graphene-ionic liquid-chitosan composite on a single-side conductive gold strip. A glassy carbon electrode, a screen-printed electrode, and a graphene-chitosan composite-modified screen-printed electrode were investigated for comparison. Scanning electron microscopy was used to characterize th2018-01-01T00:00:00Z2018 Sept.2025-04-04T23:36:20.056Z2019-03-26T23:57:14ZS-EPMC61374213017863910.1631/jzus.b170051010.1631/jzus.B1700510