biostudies-literatureHagen SENCRR NIH HHSNational Institutes of HealthNIGMS NIH HHS8245-8248https://www.ebi.ac.uk/biostudies/studies/S-EPMC6138586biostudies-literatureUnknown16(37)Two novel cyclic quaternary amine crosslinking probes are synthesized for structural mass spectrometry of protein complexes in solution and for analysis of protein interactions in organellar and whole cell extracts. Each exhibits high aqueous solubility, excellent protein crosslinking efficiencies, low collision induced dissociation (CID) energy fragmentation efficiencies, high stoichiometries of reaction, increased charges of crosslinked peptide ions, and maintenance of overall surface charge balance of crosslinked proteins.Organic & biomolecular chemistrySynthesis of CID-cleavable protein crosslinking agents containing quaternary amines for structural mass spectrometry.PMC6138586R01GM095832R01 GM095832R01 GM105942R01 GM105920R01GM105942R01GM105920R01 GM109896R01GM109896P41 RR018627Showalter HDLiu KAndrews PCJin YPiersimoni LHagen SEfalseSynthesis of CID-cleavable protein crosslinking agents containing quaternary amines for structural mass spectrometry.Two novel cyclic quaternary amine crosslinking probes are synthesized for structural mass spectrometry of protein complexes in solution and for analysis of protein interactions in organellar and whole cell extracts. Each exhibits high aqueous solubility, excellent protein crosslinking efficiencies, low collision induced dissociation (CID) energy fragmentation efficiencies, high stoichiometries of reaction, increased charges of crosslinked peptide ions, and maintenance of overall surface charge balance of crosslinked proteins.2018-01-01T00:00:00Z2018 Sep2025-04-04T02:29:13.469Z2019-10-11T07:16:09ZS-EPMC61385862953704210.1039/c8ob00329g