{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["115(37)"],"submitter":["Servili E"],"pubmed_abstract":["Depolarization-induced signaling to the nucleus by the L-type voltage-gated calcium channel Cav1.2 is widely assumed to proceed by elevating intracellular calcium. The apparent lack of quantitative correlation between Ca<sup>2+</sup> influx and gene activation suggests an alternative activation pathway. Here, we demonstrate that membrane depolarization of HEK293 cells transfected with α<sub>1</sub>1.2/β2b/α2δ subunits (Cav1.2) triggers c-Fos and MeCP2 activation via the Ras/ERK/CREB pathway. Nuclear signaling is lost either by absence of the intracellular β2 subunit or by transfecting the cells with the channel mutant α<sub>1</sub>1.2<sup>W440A</sup>/β2b/α2δ, a mutation that disrupts the interaction between α<sub>1</sub>1.2 and β2 subunits. Pulldown assays in neuronal SH-SY5Y cells and in "],"journal":["Proceedings of the National Academy of Sciences of the United States of America"],"pagination":["E8624-E8633"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6140482"],"repository":["biostudies-literature"],"pubmed_title":["β-Subunit of the voltage-gated Ca<sup>2+</sup> channel Cav1.2 drives signaling to the nucleus via H-Ras."],"pmcid":["PMC6140482"],"pubmed_authors":["Servili E","Atlas D","Trus M","Maayan D"],"additional_accession":[]},"is_claimable":false,"name":"β-Subunit of the voltage-gated Ca<sup>2+</sup> channel Cav1.2 drives signaling to the nucleus via H-Ras.","description":"Depolarization-induced signaling to the nucleus by the L-type voltage-gated calcium channel Cav1.2 is widely assumed to proceed by elevating intracellular calcium. The apparent lack of quantitative correlation between Ca<sup>2+</sup> influx and gene activation suggests an alternative activation pathway. Here, we demonstrate that membrane depolarization of HEK293 cells transfected with α<sub>1</sub>1.2/β2b/α2δ subunits (Cav1.2) triggers c-Fos and MeCP2 activation via the Ras/ERK/CREB pathway. Nuclear signaling is lost either by absence of the intracellular β2 subunit or by transfecting the cells with the channel mutant α<sub>1</sub>1.2<sup>W440A</sup>/β2b/α2δ, a mutation that disrupts the interaction between α<sub>1</sub>1.2 and β2 subunits. Pulldown assays in neuronal SH-SY5Y cells and in ","dates":{"release":"2018-01-01T00:00:00Z","publication":"2018 Sep","modification":"2026-06-08T04:21:23.4Z","creation":"2019-08-04T07:25:53Z"},"accession":"S-EPMC6140482","cross_references":{"pubmed":["30150369"],"doi":["10.1073/pnas.1805380115"]}}