biostudies-literatureSong CSpecial Fund of Xinyang Normal UniversityNational Natural Science Foundation of ChinaProgram of National Beef Cattle and Yak Industrial Technology System591-604https://www.ebi.ac.uk/biostudies/studies/S-EPMC6140903biostudies-literatureUnknown13(6)Long noncoding RNAs (lncRNAs) are involved in the regulation of skeletal muscle development. In the present study, differentially expressed lncRNAs were identified from RNA-seq data derived from myoblasts and myotubes. We conducted studies to elucidate the function and molecular mechanism of action of Linc-smad7 during skeletal muscle development. Our findings show that Linc-smad7 is upregulated during the early phase of myoblasts differentiation. In in vitro studies, we showed that overexpression of Linc-smad7 promoted the arrest of myoblasts in G1 phase, inhibited DNA replication, and induced myoblast differentiation. Our in vivo studies suggest that Linc-smad7 stimulates skeletal muscle regeneration in cardiotoxin-induced muscle injury. Mechanistically, Linc-smad7 overexpression increasEpigeneticsLinc-smad7 promotes myoblast differentiation and muscle regeneration via sponging miR-125b.PMC61409032017001CARS-3831772574Dong DLi HYang ZYang JHuang YSong CMa YChen HWang JPlath MfalseLinc-smad7 promotes myoblast differentiation and muscle regeneration via sponging miR-125b.Long noncoding RNAs (lncRNAs) are involved in the regulation of skeletal muscle development. In the present study, differentially expressed lncRNAs were identified from RNA-seq data derived from myoblasts and myotubes. We conducted studies to elucidate the function and molecular mechanism of action of Linc-smad7 during skeletal muscle development. Our findings show that Linc-smad7 is upregulated during the early phase of myoblasts differentiation. In in vitro studies, we showed that overexpression of Linc-smad7 promoted the arrest of myoblasts in G1 phase, inhibited DNA replication, and induced myoblast differentiation. Our in vivo studies suggest that Linc-smad7 stimulates skeletal muscle regeneration in cardiotoxin-induced muscle injury. Mechanistically, Linc-smad7 overexpression increas2018-01-01T00:00:00Z20182025-04-04T09:27:07.882Z2019-08-09T07:02:25ZS-EPMC61409032991261910.1080/15592294.2018.1481705