biostudies-literatureJarett JKU.S. Department of EnergyNational Science Foundation161https://www.ebi.ac.uk/biostudies/studies/S-EPMC6142677biostudies-literatureUnknown6(1)<h4>Background</h4>Nanoarchaeota are obligate symbionts of other Archaea first discovered 16 years ago, yet little is known about this largely uncultivated taxon. While Nanoarchaeota diversity has been detected in a variety of habitats using 16S rRNA gene surveys, genome sequences have been available for only three Nanoarchaeota and their hosts. The host range and adaptation of Nanoarchaeota to a wide range of environmental conditions has thus largely remained elusive. Single-cell genomics is an ideal approach to address these questions as Nanoarchaeota can be isolated while still attached to putative hosts, enabling the exploration of cell-cell interactions and fine-scale genomic diversity.<h4>Results</h4>From 22 single amplified genomes (SAGs) from three hot springs in Yellowstone NationMicrobiomeSingle-cell genomics of co-sorted Nanoarchaeota suggests novel putative host associations and diversification of proteins involved in symbiosis.PMC6142677DE-AC02-05CH11231DEB-1441717OCe-1335810Malmstrom RRJarett JKNayfach SPodar MStepanauskas RBeam JPKyrpides NCInskeep WWoyke TIvanova NNSchulz FJay ZJYoung MMunson-McGee JfalseSingle-cell genomics of co-sorted Nanoarchaeota suggests novel putative host associations and diversification of proteins involved in symbiosis.<h4>Background</h4>Nanoarchaeota are obligate symbionts of other Archaea first discovered 16 years ago, yet little is known about this largely uncultivated taxon. While Nanoarchaeota diversity has been detected in a variety of habitats using 16S rRNA gene surveys, genome sequences have been available for only three Nanoarchaeota and their hosts. The host range and adaptation of Nanoarchaeota to a wide range of environmental conditions has thus largely remained elusive. Single-cell genomics is an ideal approach to address these questions as Nanoarchaeota can be isolated while still attached to putative hosts, enabling the exploration of cell-cell interactions and fine-scale genomic diversity.<h4>Results</h4>From 22 single amplified genomes (SAGs) from three hot springs in Yellowstone Nation2018-01-01T00:00:00Z2018 Sep2026-06-06T20:41:26.967Z2019-03-26T23:56:49ZS-EPMC61426773022388910.1186/s40168-018-0539-8