{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Inci F"],"funding":["National Institute of Justice"],"pagination":["1800121"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6145299"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["5(9)"],"pubmed_abstract":["One out of every six American women has been the victim of a sexual assault in their lifetime. However, the DNA casework backlog continues to increase outpacing the nation's capacity since DNA evidence processing in sexual assault casework remains a bottleneck due to laborious and time-consuming differential extraction of victim's and perpetrator's cells. Additionally, a significant amount (60-90%) of male DNA evidence may be lost with existing procedures. Here, a microfluidic method is developed that selectively captures sperm using a unique oligosaccharide sequence (Sialyl-Lewis<sup>X</sup>), a major carbohydrate ligand for sperm-egg binding. This method is validated with forensic mock samples dating back to 2003, resulting in 70-92% sperm capture efficiency and a 60-92% reduction in epi"],"journal":["Advanced science (Weinheim, Baden-Wurttemberg, Germany)"],"pubmed_title":["A Novel On-Chip Method for Differential Extraction of Sperm in Forensic Cases."],"pmcid":["PMC6145299"],"funding_grant_id":["2017‐NE‐BX‐0004","2015‐DN‐BX‐K037"],"pubmed_authors":["Duncan G","Cimen D","Miansari M","Yesil Y","Durmus NG","Kumar DK","Filippini C","Ozen MO","Klevan L","Dhara R","Chinnasamy T","Calamak S","Yuksekkaya M","Demirci U","Inci F","Saylan Y"],"additional_accession":[]},"is_claimable":false,"name":"A Novel On-Chip Method for Differential Extraction of Sperm in Forensic Cases.","description":"One out of every six American women has been the victim of a sexual assault in their lifetime. However, the DNA casework backlog continues to increase outpacing the nation's capacity since DNA evidence processing in sexual assault casework remains a bottleneck due to laborious and time-consuming differential extraction of victim's and perpetrator's cells. Additionally, a significant amount (60-90%) of male DNA evidence may be lost with existing procedures. Here, a microfluidic method is developed that selectively captures sperm using a unique oligosaccharide sequence (Sialyl-Lewis<sup>X</sup>), a major carbohydrate ligand for sperm-egg binding. This method is validated with forensic mock samples dating back to 2003, resulting in 70-92% sperm capture efficiency and a 60-92% reduction in epi","dates":{"release":"2018-01-01T00:00:00Z","publication":"2018 Sep","modification":"2026-04-17T11:14:41.128Z","creation":"2019-03-26T23:57:22Z"},"accession":"S-EPMC6145299","cross_references":{"pubmed":["30250782"],"doi":["10.1002/advs.201800121"]}}