biostudies-literatureInci FNational Institute of Justice1800121https://www.ebi.ac.uk/biostudies/studies/S-EPMC6145299biostudies-literatureUnknown5(9)One out of every six American women has been the victim of a sexual assault in their lifetime. However, the DNA casework backlog continues to increase outpacing the nation's capacity since DNA evidence processing in sexual assault casework remains a bottleneck due to laborious and time-consuming differential extraction of victim's and perpetrator's cells. Additionally, a significant amount (60-90%) of male DNA evidence may be lost with existing procedures. Here, a microfluidic method is developed that selectively captures sperm using a unique oligosaccharide sequence (Sialyl-Lewis^X), a major carbohydrate ligand for sperm-egg binding. This method is validated with forensic mock samples dating back to 2003, resulting in 70-92% sperm capture efficiency and a 60-92% reduction in epiAdvanced science (Weinheim, Baden-Wurttemberg, Germany)A Novel On-Chip Method for Differential Extraction of Sperm in Forensic Cases.PMC61452992017‐NE‐BX‐00042015‐DN‐BX‐K037Duncan GCimen DMiansari MYesil YDurmus NGKumar DKFilippini COzen MOKlevan LDhara RChinnasamy TCalamak SYuksekkaya MDemirci UInci FSaylan YfalseA Novel On-Chip Method for Differential Extraction of Sperm in Forensic Cases.One out of every six American women has been the victim of a sexual assault in their lifetime. However, the DNA casework backlog continues to increase outpacing the nation's capacity since DNA evidence processing in sexual assault casework remains a bottleneck due to laborious and time-consuming differential extraction of victim's and perpetrator's cells. Additionally, a significant amount (60-90%) of male DNA evidence may be lost with existing procedures. Here, a microfluidic method is developed that selectively captures sperm using a unique oligosaccharide sequence (Sialyl-Lewis^X), a major carbohydrate ligand for sperm-egg binding. This method is validated with forensic mock samples dating back to 2003, resulting in 70-92% sperm capture efficiency and a 60-92% reduction in epi2018-01-01T00:00:00Z2018 Sep2026-04-17T11:14:41.128Z2019-03-26T23:57:22ZS-EPMC61452993025078210.1002/advs.201800121