{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Shi H"],"funding":["NIAID NIH HHS","National Institutes of Health"],"pagination":["50-56"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6147148"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["224"],"pubmed_abstract":["We previously established an in vitro differentiation system based on the inducible expression of the RNA binding protein 6 (RBP6), which initiated differentiation of Trypanosoma brucei non-infectious procyclics to infectious metacyclics (MFs). However, further differentiation to bloodstream forms (BFs) required infection of mice. Here we report the serendipitous isolation of a single point mutation in RBP6 (Q109K), whose expression not only generated MFs, but purified MFs continued the developmental cycle in vitro to BFs expressing variant surface glycoprotein-2 (VSG-2), formerly known as VSG 221. This transition occurred over a period of 11 days and by RNA-Seq, VSG-2 was first measureable on day 1, whereas metacyclic VSGs were detected up to 8 days. We further showed that inducible expre"],"journal":["Molecular and biochemical parasitology"],"pubmed_title":["A single-point mutation in the RNA-binding protein 6 generates Trypanosoma brucei metacyclics that are able to progress to bloodstream forms in vitro."],"pmcid":["PMC6147148"],"funding_grant_id":["R37 AI028798","R01 AI110325","AI110325","AI028798","R01 AI028798"],"pubmed_authors":["Tschudi C","Butler K","Shi H"],"additional_accession":[]},"is_claimable":false,"name":"A single-point mutation in the RNA-binding protein 6 generates Trypanosoma brucei metacyclics that are able to progress to bloodstream forms in vitro.","description":"We previously established an in vitro differentiation system based on the inducible expression of the RNA binding protein 6 (RBP6), which initiated differentiation of Trypanosoma brucei non-infectious procyclics to infectious metacyclics (MFs). However, further differentiation to bloodstream forms (BFs) required infection of mice. Here we report the serendipitous isolation of a single point mutation in RBP6 (Q109K), whose expression not only generated MFs, but purified MFs continued the developmental cycle in vitro to BFs expressing variant surface glycoprotein-2 (VSG-2), formerly known as VSG 221. This transition occurred over a period of 11 days and by RNA-Seq, VSG-2 was first measureable on day 1, whereas metacyclic VSGs were detected up to 8 days. We further showed that inducible expre","dates":{"release":"2018-01-01T00:00:00Z","publication":"2018 Sep","modification":"2026-04-07T19:27:44.408Z","creation":"2019-09-04T07:02:38Z"},"accession":"S-EPMC6147148","cross_references":{"pubmed":["30055184"],"doi":["10.1016/j.molbiopara.2018.07.011"]}}