{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Payne SR"],"funding":["National Interagency Confederation for Biological Research","Intramural NIH HHS","National Cancer Institute","NIH"],"pagination":["1059-1066.e4"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6151150"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["25(9)"],"pubmed_abstract":["In response to environmental and other stresses, the σ<sup>54</sup> subunit of bacterial RNA polymerase (RNAP) controls expression of several genes that play a significant role in the virulence of both plant and animal pathogens. Recruitment of σ<sup>54</sup> to RNAP initiates promoter-specific transcription via the double-stranded DNA denaturation mechanism of the cofactor. The RpoN box, a recognition helix found in the C-terminal region of σ<sup>54</sup>, has been identified as the component necessary for major groove insertion at the -24 position of the promoter. We employed the hydrocarbon stapled peptide methodology to design and synthesize stapled σ<sup>54</sup> peptides capable of penetrating Gram-negative bacteria, binding the σ<sup>54</sup> promoter, and blocking the interaction b"],"journal":["Cell chemical biology"],"pubmed_title":["Inhibition of Bacterial Gene Transcription with an RpoN-Based Stapled Peptide."],"pmcid":["PMC6151150"],"funding_grant_id":["ZIA BC011655"],"pubmed_authors":["Payne SR","Pau DI","Boddy CN","Kim YJ","Pharoah BM","Bernal F","Moi C","Whiting AL"],"additional_accession":[]},"is_claimable":false,"name":"Inhibition of Bacterial Gene Transcription with an RpoN-Based Stapled Peptide.","description":"In response to environmental and other stresses, the σ<sup>54</sup> subunit of bacterial RNA polymerase (RNAP) controls expression of several genes that play a significant role in the virulence of both plant and animal pathogens. Recruitment of σ<sup>54</sup> to RNAP initiates promoter-specific transcription via the double-stranded DNA denaturation mechanism of the cofactor. The RpoN box, a recognition helix found in the C-terminal region of σ<sup>54</sup>, has been identified as the component necessary for major groove insertion at the -24 position of the promoter. We employed the hydrocarbon stapled peptide methodology to design and synthesize stapled σ<sup>54</sup> peptides capable of penetrating Gram-negative bacteria, binding the σ<sup>54</sup> promoter, and blocking the interaction b","dates":{"release":"2018-01-01T00:00:00Z","publication":"2018 Sep","modification":"2026-05-03T03:01:12.473Z","creation":"2019-09-26T07:00:32Z"},"accession":"S-EPMC6151150","cross_references":{"pubmed":["29887265"],"doi":["10.1016/j.chembiol.2018.05.007"]}}