biostudies-literatureUnknown102(20)Nikolay AMax Planck Institute for Dynamics of Complex Technical SystemsA live-attenuated, human vaccine against mosquito-borne yellow fever virus has been available since the 1930s. The vaccine provides long-lasting immunity and consistent mass vaccination campaigns counter viral spread. However, traditional egg-based vaccine manufacturing requires about 12 months and vaccine supplies are chronically close to shortages. In particular, for urban outbreaks, vaccine demand can be covered rarely by global stockpiling. Thus, there is an urgent need for an improved vaccine production platform, ideally transferable to other flaviviruses including Zika virus. Here, we present a proof-of-concept study regarding cell culture-based yellow fever virus 17D (YFV) and wild-type Zika virus (ZIKV) production using duck embryo-derived EB66® cells. Based on comprehensive studieApplied microbiology and biotechnology8725-8737https://www.ebi.ac.uk/biostudies/studies/S-EPMC6153634biostudies-literatureProcess intensification of EB66® cell cultivations leads to high-yield yellow fever and Zika virus production.PMC6153634Reichl ULeon ASchwamborn KGenzel YNikolay AfalseProcess intensification of EB66® cell cultivations leads to high-yield yellow fever and Zika virus production.A live-attenuated, human vaccine against mosquito-borne yellow fever virus has been available since the 1930s. The vaccine provides long-lasting immunity and consistent mass vaccination campaigns counter viral spread. However, traditional egg-based vaccine manufacturing requires about 12 months and vaccine supplies are chronically close to shortages. In particular, for urban outbreaks, vaccine demand can be covered rarely by global stockpiling. Thus, there is an urgent need for an improved vaccine production platform, ideally transferable to other flaviviruses including Zika virus. Here, we present a proof-of-concept study regarding cell culture-based yellow fever virus 17D (YFV) and wild-type Zika virus (ZIKV) production using duck embryo-derived EB66® cells. Based on comprehensive studie2018-01-01T00:00:00Z2018 Oct2026-04-30T19:25:37.79Z2025-06-01T03:47:39.303ZS-EPMC61536343009104310.1007/s00253-018-9275-z