{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Fei F"],"funding":["Postdoctoral Science Foundation of China","National Natural Science Foundation of China"],"pagination":["49"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6154427"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13"],"pubmed_abstract":["<h4>Background</h4>San-ao decoction (SAD) has been widely used in Chinese medicine against respiratory diseases, such as asthma and rhinallergosis. The bioactive compounds for such pharmacological action remain unknown.<h4>Methods</h4>We developed a methodology to isolate the bioactive compounds of SAD. The assay involved the immobilization of beta 2-adrenoceptor (<i>β</i> <sub>2</sub>-AR) onto magnetic fine particles, the capture of target compounds by the immobilized receptor, the identification of the receptor bound compounds by reversed-phase high-performance liquid chromatography coupled with tandem mass spectrometry.<h4>Results</h4>Vicenin, shaftoside, isoshaftoside, liquiritin apioside and isoliquiritin apioside were identified as <i>β</i> <sub>2</sub>-AR ligands in SAD extract. The"],"journal":["Chinese medicine"],"pubmed_title":["Rapid screening and identification of bioactive compounds specifically binding to beta 2-adrenoceptor from San-ao decoction using affinity magnetic fine particles coupled with high-performance liquid chromatography-mass spectrometry."],"pmcid":["PMC6154427"],"funding_grant_id":["21775119","21705126","2017M620467","81702832"],"pubmed_authors":["Liu J","Zhang Y","Fei F","Li Q","Sun H","Cheng X","Wang J"],"additional_accession":[]},"is_claimable":false,"name":"Rapid screening and identification of bioactive compounds specifically binding to beta 2-adrenoceptor from San-ao decoction using affinity magnetic fine particles coupled with high-performance liquid chromatography-mass spectrometry.","description":"<h4>Background</h4>San-ao decoction (SAD) has been widely used in Chinese medicine against respiratory diseases, such as asthma and rhinallergosis. The bioactive compounds for such pharmacological action remain unknown.<h4>Methods</h4>We developed a methodology to isolate the bioactive compounds of SAD. The assay involved the immobilization of beta 2-adrenoceptor (<i>β</i> <sub>2</sub>-AR) onto magnetic fine particles, the capture of target compounds by the immobilized receptor, the identification of the receptor bound compounds by reversed-phase high-performance liquid chromatography coupled with tandem mass spectrometry.<h4>Results</h4>Vicenin, shaftoside, isoshaftoside, liquiritin apioside and isoliquiritin apioside were identified as <i>β</i> <sub>2</sub>-AR ligands in SAD extract. The","dates":{"release":"2018-01-01T00:00:00Z","publication":"2018","modification":"2026-04-30T19:32:58.774Z","creation":"2026-04-15T03:09:03.039Z"},"accession":"S-EPMC6154427","cross_references":{"pubmed":["30258489"],"doi":["10.1186/s13020-018-0207-8"]}}