biostudies-literatureFei FPostdoctoral Science Foundation of ChinaNational Natural Science Foundation of China49https://www.ebi.ac.uk/biostudies/studies/S-EPMC6154427biostudies-literatureUnknown13<h4>Background</h4>San-ao decoction (SAD) has been widely used in Chinese medicine against respiratory diseases, such as asthma and rhinallergosis. The bioactive compounds for such pharmacological action remain unknown.<h4>Methods</h4>We developed a methodology to isolate the bioactive compounds of SAD. The assay involved the immobilization of beta 2-adrenoceptor (<i>β</i> ₂-AR) onto magnetic fine particles, the capture of target compounds by the immobilized receptor, the identification of the receptor bound compounds by reversed-phase high-performance liquid chromatography coupled with tandem mass spectrometry.<h4>Results</h4>Vicenin, shaftoside, isoshaftoside, liquiritin apioside and isoliquiritin apioside were identified as <i>β</i> ₂-AR ligands in SAD extract. TheChinese medicineRapid screening and identification of bioactive compounds specifically binding to beta 2-adrenoceptor from San-ao decoction using affinity magnetic fine particles coupled with high-performance liquid chromatography-mass spectrometry.PMC615442721775119217051262017M62046781702832Liu JZhang YFei FLi QSun HCheng XWang JfalseRapid screening and identification of bioactive compounds specifically binding to beta 2-adrenoceptor from San-ao decoction using affinity magnetic fine particles coupled with high-performance liquid chromatography-mass spectrometry.<h4>Background</h4>San-ao decoction (SAD) has been widely used in Chinese medicine against respiratory diseases, such as asthma and rhinallergosis. The bioactive compounds for such pharmacological action remain unknown.<h4>Methods</h4>We developed a methodology to isolate the bioactive compounds of SAD. The assay involved the immobilization of beta 2-adrenoceptor (<i>β</i> ₂-AR) onto magnetic fine particles, the capture of target compounds by the immobilized receptor, the identification of the receptor bound compounds by reversed-phase high-performance liquid chromatography coupled with tandem mass spectrometry.<h4>Results</h4>Vicenin, shaftoside, isoshaftoside, liquiritin apioside and isoliquiritin apioside were identified as <i>β</i> ₂-AR ligands in SAD extract. The2018-01-01T00:00:00Z20182026-04-30T19:32:58.774Z2026-04-15T03:09:03.039ZS-EPMC61544273025848910.1186/s13020-018-0207-8