{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Graziano F"],"funding":["European Commission (EC)","European Commission"],"pagination":["14249"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6155284"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["8(1)"],"pubmed_abstract":["We have reported that short-term stimulation of primary human monocyte-derived macrophages (MDM) with interferon-γ (IFN-γ) and tumor necrosis factor-α (TNF-α), i.e. M1 polarization, leads to a significant containment of virus replication. Here we show that M1-MDM restimulation with these cytokines 7 days after infection (M1<sup>2</sup> MDM) promoted an increased restriction of HIV-1 replication characterized by very low levels of virus production near to undetectable levels. In comparison to control and M1-MDM that were not restimulated, M1<sup>2</sup> MDM showed a stronger reduction of both total and integrated HIV DNA as well as of viral mRNA expression. M1<sup>2</sup> MDM were characterized by an upregulated expression of restriction factors acting at the level of reverse transcription "],"journal":["Scientific reports"],"pubmed_title":["Reversible Human Immunodeficiency Virus Type-1 Latency in Primary Human Monocyte-Derived Macrophages Induced by Sustained M1 Polarization."],"pmcid":["PMC6155284"],"funding_grant_id":["305938","602893"],"pubmed_authors":["Accolla RS","Poli G","Forlani G","Graziano F","Turrini F","Aimola G","Vicenzi E"],"additional_accession":[]},"is_claimable":false,"name":"Reversible Human Immunodeficiency Virus Type-1 Latency in Primary Human Monocyte-Derived Macrophages Induced by Sustained M1 Polarization.","description":"We have reported that short-term stimulation of primary human monocyte-derived macrophages (MDM) with interferon-γ (IFN-γ) and tumor necrosis factor-α (TNF-α), i.e. M1 polarization, leads to a significant containment of virus replication. Here we show that M1-MDM restimulation with these cytokines 7 days after infection (M1<sup>2</sup> MDM) promoted an increased restriction of HIV-1 replication characterized by very low levels of virus production near to undetectable levels. In comparison to control and M1-MDM that were not restimulated, M1<sup>2</sup> MDM showed a stronger reduction of both total and integrated HIV DNA as well as of viral mRNA expression. M1<sup>2</sup> MDM were characterized by an upregulated expression of restriction factors acting at the level of reverse transcription ","dates":{"release":"2018-01-01T00:00:00Z","publication":"2018 Sep","modification":"2025-04-07T11:12:43.447Z","creation":"2019-03-26T23:57:42Z"},"accession":"S-EPMC6155284","cross_references":{"pubmed":["30250078"],"doi":["10.1038/s41598-018-32451-w"]}}