{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Mori IC"],"funding":["The Ohara Foundation for Agricultural Sciences"],"pagination":["E61"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6161278"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["7(3)"],"pubmed_abstract":["Cyclic nucleotide-gated channels (CNGCs) have been postulated to contribute significantly in plant development and stress resistance. However, their electrophysiological properties remain poorly understood. Here, we characterized barley CNGC2-3 (HvCNGC2-3) by the two-electrode voltage-clamp technique in the <i>Xenopus laevis</i> oocyte heterologous expression system. Current was not observed in <i>X. laevis</i> oocytes injected with <i>HvCNGC2-3</i> complementary RNA (cRNA) in a bathing solution containing either Na⁺ or K⁺ solely, even in the presence of 8-bromoadenosine 3',5'-cyclic monophosphate (8Br-cAMP) or 8-bromoguanosine 3',5'-cyclic monophosphate (8Br-cGMP). A weakly voltage-dependent slow hyperpolarization-activated ion current was observed in the co-presence of Na⁺ and K⁺ in the "],"journal":["Plants (Basel, Switzerland)"],"pubmed_title":["A Cyclic Nucleotide-Gated Channel, HvCNGC2-3, Is Activated by the Co-Presence of Na⁺ and K⁺ and Permeable to Na⁺ and K⁺ Non-Selectively."],"pmcid":["PMC6161278"],"funding_grant_id":["N/A"],"pubmed_authors":["Mori IC","Nakahara Y","Shibasaka M","Furuichi T","Nobukiyo Y","Katsuhara M"],"additional_accession":[]},"is_claimable":false,"name":"A Cyclic Nucleotide-Gated Channel, HvCNGC2-3, Is Activated by the Co-Presence of Na⁺ and K⁺ and Permeable to Na⁺ and K⁺ Non-Selectively.","description":"Cyclic nucleotide-gated channels (CNGCs) have been postulated to contribute significantly in plant development and stress resistance. However, their electrophysiological properties remain poorly understood. Here, we characterized barley CNGC2-3 (HvCNGC2-3) by the two-electrode voltage-clamp technique in the <i>Xenopus laevis</i> oocyte heterologous expression system. Current was not observed in <i>X. laevis</i> oocytes injected with <i>HvCNGC2-3</i> complementary RNA (cRNA) in a bathing solution containing either Na⁺ or K⁺ solely, even in the presence of 8-bromoadenosine 3',5'-cyclic monophosphate (8Br-cAMP) or 8-bromoguanosine 3',5'-cyclic monophosphate (8Br-cGMP). A weakly voltage-dependent slow hyperpolarization-activated ion current was observed in the co-presence of Na⁺ and K⁺ in the ","dates":{"release":"2018-01-01T00:00:00Z","publication":"2018 Jul","modification":"2026-06-18T05:57:13.454Z","creation":"2019-03-26T23:58:30Z"},"accession":"S-EPMC6161278","cross_references":{"pubmed":["30049942"],"doi":["10.3390/plants7030061"]}}