biostudies-literatureMori ICThe Ohara Foundation for Agricultural SciencesE61https://www.ebi.ac.uk/biostudies/studies/S-EPMC6161278biostudies-literatureUnknown7(3)Cyclic nucleotide-gated channels (CNGCs) have been postulated to contribute significantly in plant development and stress resistance. However, their electrophysiological properties remain poorly understood. Here, we characterized barley CNGC2-3 (HvCNGC2-3) by the two-electrode voltage-clamp technique in the <i>Xenopus laevis</i> oocyte heterologous expression system. Current was not observed in <i>X. laevis</i> oocytes injected with <i>HvCNGC2-3</i> complementary RNA (cRNA) in a bathing solution containing either Na⁺ or K⁺ solely, even in the presence of 8-bromoadenosine 3',5'-cyclic monophosphate (8Br-cAMP) or 8-bromoguanosine 3',5'-cyclic monophosphate (8Br-cGMP). A weakly voltage-dependent slow hyperpolarization-activated ion current was observed in the co-presence of Na⁺ and K⁺ in the Plants (Basel, Switzerland)A Cyclic Nucleotide-Gated Channel, HvCNGC2-3, Is Activated by the Co-Presence of Na⁺ and K⁺ and Permeable to Na⁺ and K⁺ Non-Selectively.PMC6161278N/AMori ICNakahara YShibasaka MFuruichi TNobukiyo YKatsuhara MfalseA Cyclic Nucleotide-Gated Channel, HvCNGC2-3, Is Activated by the Co-Presence of Na⁺ and K⁺ and Permeable to Na⁺ and K⁺ Non-Selectively.Cyclic nucleotide-gated channels (CNGCs) have been postulated to contribute significantly in plant development and stress resistance. However, their electrophysiological properties remain poorly understood. Here, we characterized barley CNGC2-3 (HvCNGC2-3) by the two-electrode voltage-clamp technique in the <i>Xenopus laevis</i> oocyte heterologous expression system. Current was not observed in <i>X. laevis</i> oocytes injected with <i>HvCNGC2-3</i> complementary RNA (cRNA) in a bathing solution containing either Na⁺ or K⁺ solely, even in the presence of 8-bromoadenosine 3',5'-cyclic monophosphate (8Br-cAMP) or 8-bromoguanosine 3',5'-cyclic monophosphate (8Br-cGMP). A weakly voltage-dependent slow hyperpolarization-activated ion current was observed in the co-presence of Na⁺ and K⁺ in the 2018-01-01T00:00:00Z2018 Jul2026-06-18T05:57:13.454Z2019-03-26T23:58:30ZS-EPMC61612783004994210.3390/plants7030061