{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Chen F"],"funding":["Innovation Project of Guangxi Graduate Education","Natural Science Foundation of Guangxi Province (Guangxi Natural Science Foundation)","National Natural Science Foundation of China (NSFC)","The open program of key laboratory of Buffalo Genetics,Breeding and Reproduction Technology of Guangxi Province"],"pagination":["1875-1891"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6166679"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["17(10)"],"pubmed_abstract":["Maternal-effect genes are especially critical for early embryonic development after fertilization and until massive activation of the embryonic genome occurs. By applying a tandem mass tag (TMT)-labeled quantitative proteomics combined with RNA sequencing approach, the proteome of the buffalo was quantitatively analyzed during parthenogenesis of mature oocytes and the two-cell stage embryo. Of 1908 quantified proteins, 123 differed significantly. The transcriptome was analyzed eight stages (GV, MII, 2-cell, 4-cell, 8-cell, 16-cell, morula, blastocyst) of Buffalo using the RNA sequencing approach, and a total of 3567 unique genes were identified to be differently expressed between all consecutive stages of pre-implantation development. Validation of proteomics results (TUBB3, CTNNA1, CDH3, "],"journal":["Molecular & cellular proteomics : MCP"],"pubmed_title":["Integrated Analysis of Quantitative Proteome and Transcriptional Profiles Reveals the Dynamic Function of Maternally Expressed Proteins After Parthenogenetic Activation of Buffalo Oocyte."],"pmcid":["PMC6166679"],"funding_grant_id":["31460603","SNKF-2017–01","2014GXNSFAA118134","YCBZ2017005"],"pubmed_authors":["Fu Q","Huang F","Hou Z","Deng T","Liang X","Lu Y","Chen F","Zhang M","Zhang P","Chen D","Huang Y","Pu L","Xu Z"],"additional_accession":[]},"is_claimable":false,"name":"Integrated Analysis of Quantitative Proteome and Transcriptional Profiles Reveals the Dynamic Function of Maternally Expressed Proteins After Parthenogenetic Activation of Buffalo Oocyte.","description":"Maternal-effect genes are especially critical for early embryonic development after fertilization and until massive activation of the embryonic genome occurs. By applying a tandem mass tag (TMT)-labeled quantitative proteomics combined with RNA sequencing approach, the proteome of the buffalo was quantitatively analyzed during parthenogenesis of mature oocytes and the two-cell stage embryo. Of 1908 quantified proteins, 123 differed significantly. The transcriptome was analyzed eight stages (GV, MII, 2-cell, 4-cell, 8-cell, 16-cell, morula, blastocyst) of Buffalo using the RNA sequencing approach, and a total of 3567 unique genes were identified to be differently expressed between all consecutive stages of pre-implantation development. Validation of proteomics results (TUBB3, CTNNA1, CDH3, ","dates":{"release":"2018-01-01T00:00:00Z","publication":"2018 Oct","modification":"2026-04-17T11:31:02.149Z","creation":"2025-06-01T03:26:45.394Z"},"accession":"S-EPMC6166679","cross_references":{"pubmed":["30002204"],"doi":["10.1074/mcp.ra118.000556","10.1074/mcp.RA118.000556"]}}