biostudies-literatureNalamolu KRIllinois Neurological Institute, OSF HealthCare FoundationNIDDK NIH HHS153-159https://www.ebi.ac.uk/biostudies/studies/S-EPMC6169614biostudies-literatureUnknown3(3)<h4>Background and purpose</h4>Recent reports from our laboratory demonstrated the post-ischaemic expression profile of various matrix metalloproteinases (MMPs) in rats and the detrimental role of MMP-12 in post-stroke brain damage. We hypothesise that the post-stroke dysregulation of MMPs is similar across species and that genetic deletion of MMP-12 would not affect the post-stroke expression of other MMPs. We tested our hypothesis by determining the pre-ischaemic and post-ischaemic expression profile of MMPs in wild-type and MMP-12 knockout mice.<h4>Methods</h4>Focal cerebral ischaemia was induced in wild-type and MMP-12 knockout mice by middle cerebral artery occlusion procedure by insertion of a monofilament suture. One hour after ischaemia, reperfusion was initiated by removing the moStroke and vascular neurologyPost-stroke mRNA expression profile of MMPs: effect of genetic deletion of MMP-12.PMC6169614R01 DK104031Chelluboina BKlopfenstein JDVenkatesh IMagruder IBBoini KMPinson DMVeeravalli KKFru DNMohandass ANalamolu KRfalsePost-stroke mRNA expression profile of MMPs: effect of genetic deletion of MMP-12.<h4>Background and purpose</h4>Recent reports from our laboratory demonstrated the post-ischaemic expression profile of various matrix metalloproteinases (MMPs) in rats and the detrimental role of MMP-12 in post-stroke brain damage. We hypothesise that the post-stroke dysregulation of MMPs is similar across species and that genetic deletion of MMP-12 would not affect the post-stroke expression of other MMPs. We tested our hypothesis by determining the pre-ischaemic and post-ischaemic expression profile of MMPs in wild-type and MMP-12 knockout mice.<h4>Methods</h4>Focal cerebral ischaemia was induced in wild-type and MMP-12 knockout mice by middle cerebral artery occlusion procedure by insertion of a monofilament suture. One hour after ischaemia, reperfusion was initiated by removing the mo2018-01-01T00:00:00Z2018 Sep2025-04-04T19:42:30.154Z2021-02-19T19:53:12ZS-EPMC61696143029447110.1136/svn-2018-000142