biostudies-literatureAhmed MIMBundesministerium für Bildung und ForschungDeutsches Zentrum für InfektionsforschungEuropean & Developing Countries Clinical Trials Partnership (EDCTP)European Commission2247https://www.ebi.ac.uk/biostudies/studies/S-EPMC6172348biostudies-literatureUnknown9<b>Background:</b> The analysis of phenotypic characteristics on <i>Mycobacterium tuberculosis</i> (MTB)-specific T cells is a promising approach for the diagnosis of active tuberculosis (aTB) and for monitoring treatment success. We therefore studied phenotypic changes on MTB-specific CD4 T cells upon anti-tuberculosis treatment initiation in relation to the treatment response as determined by sputum culture. <b>Methods:</b> Peripheral blood mononuclear cells from subjects with latent MTB infection (<i>n</i> = 16) and aTB (<i>n</i> = 39) at baseline, weeks 9, 12, and 26 (end of treatment) were analyzed after intracellular interferon gamma staining and overnight stimulation with tuberculin. Liquid sputum cultures were performed weekly until week 12 and during 4 visits until week 26. <b>ResFrontiers in immunologyPhenotypic Changes on Mycobacterium Tuberculosis-Specific CD4 T Cells as Surrogate Markers for Tuberculosis Treatment Efficacy.PMC6172348IP.2007.32011.012IP.2007.32011.013ICA-CT-2002-10048IP.2007.32011.01101KA0901von Both UBoeree MJSemvua HMekota AMHeinrich NLoose RHoelscher MSaathoff EKroidl IKibiki GNtinginya NEChachage MGeldmacher CMtafya BAMtabho CHaule AAhmed MIMMpagama SGillespie SHHeld KfalsePhenotypic Changes on Mycobacterium Tuberculosis-Specific CD4 T Cells as Surrogate Markers for Tuberculosis Treatment Efficacy.<b>Background:</b> The analysis of phenotypic characteristics on <i>Mycobacterium tuberculosis</i> (MTB)-specific T cells is a promising approach for the diagnosis of active tuberculosis (aTB) and for monitoring treatment success. We therefore studied phenotypic changes on MTB-specific CD4 T cells upon anti-tuberculosis treatment initiation in relation to the treatment response as determined by sputum culture. <b>Methods:</b> Peripheral blood mononuclear cells from subjects with latent MTB infection (<i>n</i> = 16) and aTB (<i>n</i> = 39) at baseline, weeks 9, 12, and 26 (end of treatment) were analyzed after intracellular interferon gamma staining and overnight stimulation with tuberculin. Liquid sputum cultures were performed weekly until week 12 and during 4 visits until week 26. <b>Res2018-01-01T00:00:00Z20182026-04-07T15:03:38.84Z2019-03-27T00:02:59ZS-EPMC61723483032381810.3389/fimmu.2018.02247