{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Shan C"],"funding":["HHS | National Institutes of Health","NCATS NIH HHS","NIAID NIH HHS"],"pagination":["e01002-18"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC6189489"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["92(21)"],"pubmed_abstract":["Dengue virus (DENV) is the most prevalent mosquito-transmitted viral pathogen in humans. The recently licensed dengue vaccine has major weaknesses. Therefore, there is an urgent need to develop improved dengue vaccines. Here, we report a virion assembly-defective DENV as a vaccine platform. DENV containing an amino acid deletion (K188) in nonstructural protein 2A (NS2A) is fully competent in viral RNA replication but is completely defective in virion assembly. When <i>trans</i>-complemented with wild-type NS2A protein, the virion assembly defect could be rescued, generating pseudoinfectious virus (PIV<sub>NS2A</sub>) that could initiate single-round infection. The <i>trans</i>-complementation efficiency could be significantly improved through selection for adaptive mutations, leading to hi"],"journal":["Journal of virology"],"pubmed_title":["Using a Virion Assembly-Defective Dengue Virus as a Vaccine Approach."],"pmcid":["PMC6189489"],"funding_grant_id":["AI127744","R01 AI127744","UL1 TR001439"],"pubmed_authors":["Zou J","Zust R","Xie X","Fink K","Shi PY","Ambrose R","Shan C","Zhang B","Mackenzie J"],"additional_accession":[]},"is_claimable":false,"name":"Using a Virion Assembly-Defective Dengue Virus as a Vaccine Approach.","description":"Dengue virus (DENV) is the most prevalent mosquito-transmitted viral pathogen in humans. The recently licensed dengue vaccine has major weaknesses. Therefore, there is an urgent need to develop improved dengue vaccines. Here, we report a virion assembly-defective DENV as a vaccine platform. DENV containing an amino acid deletion (K188) in nonstructural protein 2A (NS2A) is fully competent in viral RNA replication but is completely defective in virion assembly. When <i>trans</i>-complemented with wild-type NS2A protein, the virion assembly defect could be rescued, generating pseudoinfectious virus (PIV<sub>NS2A</sub>) that could initiate single-round infection. The <i>trans</i>-complementation efficiency could be significantly improved through selection for adaptive mutations, leading to hi","dates":{"release":"2018-01-01T00:00:00Z","publication":"2018 Nov","modification":"2025-04-05T00:11:30.442Z","creation":"2019-06-06T20:18:21Z"},"accession":"S-EPMC6189489","cross_references":{"pubmed":["30111567"],"doi":["10.1128/JVI.01002-18","10.1128/jvi.01002-18"]}}